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PE anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A_BB7dot2_PE_100809
HLA-A2 positive (top) and HLA-A2 negative (bottom) donors peripheral blood lymphocytes stained with BB7.2 PE.
  • A_BB7dot2_PE_100809
    HLA-A2 positive (top) and HLA-A2 negative (bottom) donors peripheral blood lymphocytes stained with BB7.2 PE.
  • B_BB7dot2_PE_100809
Compare all formats See PE spectral data
Cat # Size Price Save
343305 25 tests ¥28,380
343306 100 tests ¥51,730
Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations
  1. Sun Y, et al. 2023. Sci Adv. 9:eade7151. PubMed
  2. Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed
  3. Xiao C, et al. 2023. Nat Aging. 3:418. PubMed
  4. Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed
  5. Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
  6. Schuler P, et al. 2014. Clin Cancer Res. 20:2433. PubMed
  7. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  8. Wang X, et al. 2011. Blood. 117:1888. PubMed
  9. Xiao C, et al. 2022. iScience. 25:103934. PubMed
  10. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  11. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  12. Klatt MG, et al. 2020. J Proteomics. 228:103921. PubMed
  13. Abdelaziz MO, et al. 2019. Front Immunol. 1.65. PubMed
  14. Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed
  15. Lai J, et al. 2016. Blood. 128: 1396 - 1407. PubMed
  16. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  17. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  18. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  19. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  20. Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed
  21. Pettmann J, et al. 2021. eLife. 10:00. PubMed
RRID
AB_1877227 (BioLegend Cat. No. 343305)
AB_1877227 (BioLegend Cat. No. 343306)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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