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PE/Cyanine7 anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12_PECy7_2_102110
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram) (gated on lymphoid cell population).
  • TC15-12F12_PECy7_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram) (gated on lymphoid cell population).
  • TC15-12F12dot2_PECy7_957
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
115913 25 µg ¥19,940
115914 100 µg ¥55,360
Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  2. Upadhaya S, et al. 2020. Cell Stem Cell. 27(2):336-345.e4. PubMed
  3. Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
  4. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  6. Muto T, et al. 2022. Cell Stem Cell. 29:298. PubMed
  7. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  8. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  9. Qiu J, et al. 2022. STAR Protoc. 3:101828. PubMed
  10. Feng J, et al. 2022. Immunity. 55:405. PubMed
  11. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
  12. Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed
  13. Le DT, et al. 2023. iScience. 26:106059. PubMed
  14. Muto T, et al. 2023. Haematologica. . PubMed
  15. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  16. Muto T, et al. 2020. Nat Immunol. 1.246527778. PubMed
  17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  20. Grinenko T, et al. 2018. Nat Commun. 9:1898. PubMed
  21. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  22. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  23. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  24. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  25. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  26. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  27. Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
  28. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  29. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  30. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  31. Jordan M, et al. 2011. J Immunol. 186:3953. PubMed
  32. Eisele AS, et al. 2022. Elife. 11:. PubMed
  33. Gustafsson K, et al. 2013. Exp Cell Res. 319:1852. PubMed
  34. Halene S, et al. 2010. Blood. 116:1942. PubMed
  35. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  36. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  37. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  38. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  39. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  40. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  41. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
  42. Zhong L, et al. 2022. JCI Insight. 7:. PubMed
  43. Clapes T, et al. 2021. Nat Cell Biol. 23:704. PubMed
  44. Yankova E, et al. 2021. Nature. 593:597. PubMed
  45. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  46. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  47. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  48. Maetzig T, et al. 2017. Mol Ther. 25:606. PubMed
  49. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  50. Tober J, et al. 2013. Development. 140:3765. PubMed
  51. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  52. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  53. Liang R, et al. 2020. Cell Stem Cell. 359:26. PubMed
  54. Yu W, et al. 2020. J Clin Invest. . PubMed
  55. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  56. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  57. Karamitros D, et al. 2015. Development. 142:70. PubMed
  58. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  59. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  60. Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed
  61. Xia P, et al. 2015. J Exp Med. 212: 2305 - 2321. PubMed
  62. Nestorowa S, et al. 2016. Blood. 128: e20 - e31. PubMed
  63. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  64. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  65. Glotzbach J, et al. 2011. PLoS One. 6:e21211. PubMed
  66. Young IC, et al. 2021. Cell Rep. 37:110036. PubMed
  67. Koide S, et al. 2022. iScience. 25:103603. PubMed
  68. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  69. Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed
  70. Chlon TM, et al. 2021. Cell Stem Cell. :. PubMed
  71. Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
  72. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  73. Kent D, et al. 2009. Blood. 113:6342. PubMed
  74. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  75. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  76. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  77. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  78. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  79. Guo F, et al. 2013. Haematologica. 98:1353. PubMed
  80. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  81. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  82. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  83. Fast EM, et al. 2021. Elife. 10:. PubMed
  84. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  85. Rimmelé P, et al. 2015. EMBO Rep. 16: 1164-1176. PubMed
  86. Gerhardt D, et al. 2014. Genes Dev. 28:576. PubMed
RRID
AB_439796 (BioLegend Cat. No. 115913)
AB_439796 (BioLegend Cat. No. 115914)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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