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Purified anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_Purified_HLA-DR_Antibody_012519_updated.png
Human peripheral blood lymphocytes were stained with CD19 APC and HLA-DR (clone L243) Purified (left) or Purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG FITC.
  • L243_Purified_HLA-DR_Antibody_012519_updated.png
    Human peripheral blood lymphocytes were stained with CD19 APC and HLA-DR (clone L243) Purified (left) or Purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG FITC.
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Cat # Size Price Save
307602 100 µg ¥15,840
Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IP, WB, Block, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  3. Nagamatsu T, et al. 2009. Hum Reprod. 24:3160. PubMed
  4. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  5. Law JC, et al. 2021. J Immunol. 206:37. PubMed
  6. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  7. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  8. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  9. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  10. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  11. Lu Y, et al. 2017. Cell Mol Immunol. 1.054166667. PubMed
  12. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  13. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  14. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  15. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  16. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  17. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  18. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  19. Liu R, et al. 2022. Curr Protoc. 2:e350. PubMed
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  22. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  23. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  24. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  25. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  26. Tal MC, et al. 2020. MBio. 11:00. PubMed
  27. Mirandola L, et al. 2017. Oncotarget. 8:74378. PubMed
  28. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  29. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  30. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  31. Parker R, et al. 2020. bioRxiv. . PubMed
  32. Salvat R, et al. 2014. J Vis Exp. 85: 51308. PubMed
  33. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  34. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  35. Wu Y, et al. 2018. J Immunol. 200:1627. PubMed
  36. Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed
  37. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  38. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  39. Raffray L, et al. 2020. Immunology. 159:88. PubMed
  40. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  41. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  42. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  43. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  44. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  45. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  46. Olin A, et al. 2018. Cell. 174:1277. PubMed
  47. Gravastrand CS, et al. 2019. J Immunol. 203(4). PubMed
  48. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  49. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  50. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  51. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  52. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  53. Tsuruta M, et al. 2018. Oncoimmunology. 7:e1415687. PubMed
  54. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  55. Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
  56. Henrick BM, et al. 2021. Cell. . PubMed
  57. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  58. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  59. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  60. Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
  61. Aljabri A, et al. 2019. Cell Death Dis. 0.579861111. PubMed
  62. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  63. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  64. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  65. Schwabenland M, et al. 2021. Immunity. . PubMed
  66. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  67. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  68. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  69. Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
  70. Zeiner PS, et al. 2018. Acta Neuropathol Commun. 6:18. PubMed
  71. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  72. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  73. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  74. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
  75. Scholzen A, et al. 2019. Front Immunol. 0.560416667. PubMed
  76. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  77. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  78. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  79. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_314680 (BioLegend Cat. No. 307602)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

Other Formats

View All HLA-DR Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human HLA-DR L243 FC
FITC anti-human HLA-DR L243 FC
PE anti-human HLA-DR L243 FC
PE/Cyanine5 anti-human HLA-DR L243 FC
Purified anti-human HLA-DR L243 FC,CyTOF®,IP,WB,Block,IHC-F
Biotin anti-human HLA-DR L243 FC
PE/Cyanine7 anti-human HLA-DR L243 FC
APC/Cyanine7 anti-human HLA-DR L243 FC
Alexa Fluor® 488 anti-human HLA-DR L243 FC,SB
Alexa Fluor® 647 anti-human HLA-DR L243 FC
Pacific Blue™ anti-human HLA-DR L243 FC
Alexa Fluor® 700 anti-human HLA-DR L243 FC
PerCP anti-human HLA-DR L243 FC
PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
Brilliant Violet 605™ anti-human HLA-DR L243 FC
Brilliant Violet 421™ anti-human HLA-DR L243 FC
Brilliant Violet 570™ anti-human HLA-DR L243 FC
Brilliant Violet 711™ anti-human HLA-DR L243 FC
Brilliant Violet 785™ anti-human HLA-DR L243 FC
Brilliant Violet 510™ anti-human HLA-DR L243 FC
Ultra-LEAF™ Purified anti-human HLA-DR L243 FC,CyTOF®,IP,WB,Block,IHC-F
Brilliant Violet 650™ anti-human HLA-DR L243 FC
Purified anti-human HLA-DR (Maxpar® Ready) L243 FC,CyTOF®
PE/Dazzle™ 594 anti-human HLA-DR L243 FC
FITC anti-human HLA-DR L243 FC
APC/Fire™ 750 anti-human HLA-DR L243 FC
Pacific Blue™ anti-human HLA-DR L243 FC
APC anti-human HLA-DR L243 FC
PE/Dazzle™ 594 anti-human HLA-DR L243 FC
PE/Cyanine7 anti-human HLA-DR L243 FC
TotalSeq™-A0159 anti-human HLA-DR L243 PG
TotalSeq™-B0159 anti-human HLA-DR L243 PG
TotalSeq™-C0159 anti-human HLA-DR L243 PG
Brilliant Violet 750™ anti-human HLA-DR L243 FC
APC/Fire™ 750 anti-human HLA-DR L243 FC
PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
APC/Fire™ 810 anti-human HLA-DR L243 FC
PE/Fire™ 640 anti-human HLA-DR L243 FC
PE anti-human HLA-DR L243 FC
Spark Violet™ 538 anti-human HLA-DR Antibody L243 FC
KIRAVIA Blue 520™ anti-human HLA-DR L243 FC
TotalSeq™-D0159 anti-human HLA-DR L243 PG
PE/Fire™ 810 anti-human HLA-DR L243 FC
GMP PE/Dazzle™ 594 anti-human HLA-DR L243 FC
Spark Violet™ 423 anti-human HLA-DR L243 FC
PerCP anti-human HLA-DR L243 FC
GMP FITC anti-human HLA-DR L243 FC
GMP APC anti-human HLA-DR L243 FC
GMP PE/Cyanine7 anti-human HLA-DR L243 FC
GMP Pacific Blue™ anti-human HLA-DR L243 FC
GMP APC/Fire™ 750 anti-human HLA-DR L243 FC
Spark Violet™ 500 anti-human HLA-DR L243 FC
GMP PerCP/Cyanine5.5 anti-human HLA-DR L243 FC
GMP PE anti-human HLA-DR L243 FC
Spark UV™ 387 anti-human HLA-DR L243 FC
Spark Blue™ 515 anti-human HLA-DR L243 FC
Spark NIR™ 685 anti-human HLA-DR L243 FC
PerCP/Fire™ 806 anti-human HLA-DR L243 FC
PE/Fire™ 700 anti-human HLA-DR L243 FC
Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™) L243 FC
Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™) L243 FC
PE/Fire™ 744 anti-human HLA-DR L243 FC
Spark PLUS UV395™ anti-human HLA-DR L243 FC
Spark Violet™ 423 anti-human HLA-DR L243 FC
Go To Top Version: 3    Revision Date: 01/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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