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Purified anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
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Cat # Size Price Save
123101 50 µg ¥10,340
123102 500 µg ¥45,930
Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  2. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  3. Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed
  4. Elshal M, et al. 2022. Arch Pharm Res. . PubMed
  5. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  6. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  7. Volmari A, et al. 2021. Hepatol Commun. 5:2104. PubMed
  8. Li F, et al. 2020. EMBO J. 39:e103205. PubMed
  9. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  10. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  11. Marneros AG, et al. 2023. FEBS J. 290:28. PubMed
  12. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  13. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  14. Fu A, et al. 2022. Cell. 185:1356. PubMed
  15. Marquardt RM, et al. 2022. Int J Mol Sci. 23: . PubMed
  16. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  17. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  18. Rehrauer H, et al. 2018. Oncogene. 7:1487250. PubMed
  19. Gomes AC, et al. 2019. J Immunol. 203:2485. PubMed
  20. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  21. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  22. Cheng AA, et al. 2021. Am J Physiol Endocrinol Metab. 320:E438. PubMed
  23. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  24. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  25. Maestro S, et al. 2021. Viruses. 13: . PubMed
  26. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  27. Kaneko T, et al. 2012. J Immunol. 188:5397. PubMed
  28. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  29. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  30. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  31. Gray ME, et al. 2018. J Biomed Mater Res B Appl Biomater. 107(5):1620-1633. PubMed
  32. Wang B et al. 2018. Cell stem cell. 22(2):206-220 . PubMed
  33. Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed
  34. Accarias S, et al. 2020. J Cell Sci. 133:. PubMed
  35. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  36. Lu L, et al. 2022. Sci Rep. 12:931. PubMed
  37. Yang J, et al. 2022. Biosci Rep. :. PubMed
  38. Elshal M, et al. 2022. Inflammopharmacology. :. PubMed
  39. Chen YS, et al. 2019. Cells. 8:3. PubMed
  40. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  41. Karnezis T et al. 2019. Cancer Res. 79(7):1558-1572 . PubMed
  42. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  43. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  44. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  45. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  46. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  47. Xu Q, et al. 2021. Front Physiol. 12:732084. PubMed
  48. Kennedy CL, et al. 2021. Toxics. 9:. PubMed
  49. Goswami S, et al. 2021. J Cell Physiol. 236:8148. PubMed
  50. Cai Z, et al. 2021. Front Immunol. 12:646384. PubMed
  51. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  52. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  53. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  54. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  55. Chen J, et al. 2018. Hypertension. 71:877. PubMed
  56. Weis S et al. 2017. Cell. 169(7):1263-1275 . PubMed
  57. Cvetanova B, et al. 2021. Int J Mol Sci. 22: . PubMed
  58. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  59. Huang YL, et al. 2021. Antioxidants (Basel). 10:. PubMed
  60. Peng J, et al. 2020. Toxicol Res (Camb). 9:609. PubMed
  61. Huang YL, et al. 2021. Aging Cell. 20:e13523. PubMed
  62. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  63. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  64. Malsy J, et al. 2020. Elife. 9:00. PubMed
  65. Cozzitorto C, et al. 2020. Developmental Cell. 55(2):150-162.e6. PubMed
  66. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  67. Ma F, et al. 2020. Sci Signal. :13. PubMed
  68. Motoyama S, et al. 2020. Cells. :9. PubMed
  69. Cheng Y, et al. 2018. J Exp Med. 215:2919. PubMed
  70. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  71. Duarte N, et al. 2018. Hepatol Commun. 0.833333333. PubMed
  72. Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed
  73. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  74. Miyake M, et al. 2021. iScience. 24:103448. PubMed
  75. Billerhart M, et al. 2021. Mol Ther Oncolytics. 23:192. PubMed
  76. Kuziel G, et al. 2020. Cancers (Basel). 12:00. PubMed
  77. Hayakawa K, et al. 2016. J Cereb Blood Flow Metab. 36: 781-793. PubMed
  78. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  79. Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
  80. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  81. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  82. Matsuhira T, et al. 2021. Biochem Biophys Rep. 28:101118. PubMed
  83. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  84. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  85. Bálint L, et al. 2020. Front Immunol. 2.529861111. PubMed
  86. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  87. Matsuo R, et al. 2022. JID Innov. 2:100141. PubMed
  88. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  89. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  90. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  92. Cassidy LD, et al. 2018. Autophagy. 1.455555556. PubMed
  93. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  94. Tsujinaka H, et al. 2020. Nat Commun. 0.940277778. PubMed
  95. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  96. Rätsep MT, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 315:L977. PubMed
  97. Sato Y, et al. 2021. BMC Cancer. 21:1222. PubMed
  98. Wang Y, et al. 2021. Sci Transl Med. 13:. PubMed
  99. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  100. Yang K, et al. 2017. Nature. 548:602. PubMed
  101. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  102. Kobayashi M, et al. 2008. J Leukoc Biol. 83:1354. PubMed
  103. Andersson &, et al. 2011. Clin Cancer Res. 3.25. PubMed
  104. Kaufmann B, et al. 2022. EMBO Rep. 23:e54446. PubMed
  105. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  106. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  107. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  108. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  109. Mueller–Ortiz SL, et al. 2019. J Immunol. 203:2701. PubMed
  110. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  111. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
  112. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  113. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  114. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  115. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  116. Janardhan KS, et al. 2018. Toxicol Pathol. 46:488. PubMed
  117. Defaye M, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:977. PubMed
  118. Ejima R, et al. 2021. Nutrients. 13:. PubMed
  119. Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed
  120. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  121. Piao Y, et al. 2016. Neuro Oncology. 18: 1230 - 1241. PubMed
  122. Poeckel D, et al. 2009. J Biol Chem. 284:21077. PubMed
  123. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  124. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  125. Wu Y, et al. 2020. Biol Reprod. 1213:102. PubMed
  126. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  127. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  128. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  129. Jokinen MP, et al. 2017. Toxicol Pathol. 45:774. PubMed
  130. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  131. Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed
  132. Hosomi K, et al. 2022. Nat Commun. 13:4477. PubMed
  133. Seo SU, et al. 2021. Front Immunol. 12:697162. PubMed
  134. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  135. Sasi S, et al. 2014. J Biol Chem. 289:14178. PubMed
  136. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  137. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  138. Burns K, et al. 2012. Endocrinology. 153:3960. PubMed
  139. Toyohara T, et al. 2020. Cell Stem Cell. 27:147. PubMed
  140. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  141. Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
  142. Wang Y, et al. 2019. Biomed Res Int. 2019:5756189. PubMed
  143. Hong JE, et al. 2019. Front Microbiol. 0.727083333. PubMed
  144. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  145. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  146. Liu J, et al. 2022. Small. 18:e2106172. PubMed
  147. Li X, et al. 2021. Eur J Immunol. 51:2452. PubMed
  148. Yang CC, et al. 2021. Cancers (Basel). 13:. PubMed
  149. Um HN, et al. 2021. Cell Death Dis. 12:243. PubMed
  150. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  151. McArthur S, et al. 2020. J Clin Invest. 130:1156. PubMed
  152. Dyrna F, et al. 2018. Am J Sports Med. 46:3511. PubMed
  153. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  154. Yi X, et al. 2021. J Bone Miner Res. 36:2426. PubMed
  155. Thakur V, et al. 2014. Toxicol Lett. 230:322. PubMed
  156. Quilichini E, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.671527778. PubMed
  157. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  158. Hunt LC, et al. 2021. Cell Rep. 37:109971. PubMed
  159. Nagatake T, et al. 2021. Sci Rep. 11:10426. PubMed
  160. Wang Y, et al. 2021. Nat Commun. 0.570833333. PubMed
  161. Kikuchi N, et al. 2011. Am J Respir Cell Mol Biol. 45:600. PubMed
  162. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  163. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  164. Dubik M, et al. 2021. Front Neurosci. 15:682451. PubMed
  165. Baluk P, et al. 2022. Methods Mol Biol. 2441:115. PubMed
  166. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  167. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  168. Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
  169. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
  170. Campbell KM, et al. 2019. Cell Rep. 28:1526. PubMed
  171. Ireland L, et al. 2018. Oncogene. 293:4244. PubMed
RRID
AB_893504 (BioLegend Cat. No. 123101)
AB_893504 (BioLegend Cat. No. 123102)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 2    Revision Date: 10/25/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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