Purified anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Raised against granulocytes of mouse origin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The Ly-6G/Ly-6C antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁹. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁹. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4²⁰.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity², in vivo depletion^3-5,9, immunoprecipitation¹, immunohistochemical staining⁶ (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections¹¹ and zinc-fixed sections¹⁵), and Western blotting⁷. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)²⁰.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)

Product Citations

1. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
2. Ramirez-Moral I, et al. 2021. J Pathol. 253:374. PubMed
3. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
4. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
5. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
6. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
7. Li L, et al. 2023. Br J Pharmacol. 180:287. PubMed
8. Ruiz-Fernández de Córdoba B, et al. 2022. Cancer Discov. 12:1356. PubMed
9. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
10. Matsuno Y, et al. 2007. Am J Respir Crit Care Med. 176:1015. PubMed
11. Weidner H, et al. 2020. JCI Insight. 5:00. PubMed
12. Li Y, et al. 2022. Int J Biol Sci. 18:3697. PubMed
13. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
14. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
15. Nederhoff MGJ, et al. 2019. Auton Neurosci. 221:102580. PubMed
16. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
17. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
18. Zou C, et al. 2020. Cell Rep. 33:108447. PubMed
19. Yang J, et al. 2022. Biosci Rep. :. PubMed
20. Hameed AM, et al. 2020. Sci Rep. 5.229166667. PubMed
21. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
22. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
23. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
24. Zalacain M, et al. 2021. Mol Ther Oncolytics. 20:23. PubMed
25. Ronkina N, et al. 2019. J Immunol. 203:2291. PubMed
26. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
27. Bernal S, et al. 2020. British Journal of Pharmacology. 178(3):564-581. PubMed
28. Lopez-Sanz L, et al. 2021. Clinical and Translational Medicine. 11(7):e463. PubMed
29. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
30. Gautam J, et al. 2020. J Neuroinflammation. 17:103. PubMed
31. McCormick B, et al. 2019. J Immunol. 203:1579. PubMed
32. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
33. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
34. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
35. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
36. Pérez-Hernández M, et al. 2021. Front Physiol. 11:623190. PubMed
37. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
38. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
39. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
40. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
41. Wang H, et al. 2021. Nat Commun. 12:4826. PubMed
42. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
43. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
44. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
45. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
46. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
47. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
48. Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed
49. Arnold S, et al. 2008. Exp Biol Med. 233:860. PubMed
50. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
51. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
52. Wicki S, et al. 2018. Int J Mol Sci. 1.266666667. PubMed
53. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
54. Enders M, et al. 2020. J Immunol. 204:87. PubMed
55. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
56. Piao Y, et al. 2012. Neuro Oncology. 14:1379. PubMed
57. Artz A, Butz S, Vestweber D 2016. Am J Physiol Renal Physiol. 311: F176 - F181. PubMed
58. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
59. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
60. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
61. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
62. Wang SS, et al. 2021. Cell Chemical Biology. 28(5):699-710.e5. PubMed
63. Wu Y, et al. 2020. Biol Reprod. 1213:102. PubMed
64. Tsukui H, et al. 2020. BMC Cancer. 20:411. PubMed
65. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
66. Ritchie N, Ijaz U, and Evans T. 2017. BMC Genomics.. 10.1186/s12864-017-4215-3. PubMed
67. Nguyen CH, et al. 2020. Biomedicines. 8:. PubMed
68. Bogorodskiy AO, et al. 2020. Front Immunol. 11:298. PubMed
69. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
70. Ren AQ, et al. 2021. Front Pharmacol. 12:693298. PubMed
71. Ruiz-Fernandez de Cordoba B, et al. 2022. Cancer Discov. . PubMed
72. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
73. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
74. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
75. Hwang BJ, et al. 2019. Oncogene. 38:7491. PubMed
76. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
77. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
78. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
79. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
80. Jin H, et al. 2021. Int J Mol Sci. 22:. PubMed
81. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
82. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
83. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
84. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
85. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
86. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
87. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
88. Dubik M, et al. 2021. Front Neurosci. 15:682451. PubMed
89. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
90. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
91. Sparber F et al. 2019. British journal of pharmacology. 176(11):1728-1744 . PubMed
92. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed

RRID

AB_313366 (BioLegend Cat. No. 108401)
AB_313366 (BioLegend Cat. No. 108402)

Antigen Details

Structure

21-25 kD

Distribution

Granulocytes, monocytes

Cell Type

Granulocytes, Monocytes, Neutrophils

Biology Area

Immunology, Innate Immunity

Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID

17067 View all products for this Gene ID 546644 View all products for this Gene ID

UniProt

View information about Ly-6G Ly-6C on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis