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Recombinant Mouse IL-23 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukine 23 (Interleukin 23, alpha subunit p19 and Interleukin-12 subunit beta p40)
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Product Citations
publications
Mouse_IL-23_092210
Mouse IL-23 induces IL-17A in mouse splenocytes.
  • Mouse_IL-23_092210
    Mouse IL-23 induces IL-17A in mouse splenocytes.
  • Mouse_IL-23_RECOM_BA_020923
    Recombinant mouse IL-23 induces production of IL-17A in mouse splenocytes in a dose dependent manner. BioLegend’s protein was compared side-by-side to the leading competitor’s equivalent product.
Cat # Size Price Save
589002 10 µg ¥64,280
589004 25 µg ¥134,330
589006 100 µg ¥419,700
589008 500 µg ¥1,259,080
Description

IL-23 is a member of the IL-6 family of cytokines, and it is constituted by two subunits, p19 and p40. The p19-p40 heterodimer is stabilized by a disulfide bond. The subunit p40 is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells; nevertheless, p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rb1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription signaling molecules (STAT). JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.

Product Details
Technical data sheet

Product Details

Source
The mouse IL-23 consists of two subunits linked via a disulphide bond: P19 (Accession# NM_031252: Met1-Ala 196) and P40 (Accession# NM_008352 : Met Met1-Ser 335). Mouse IL-23 was expressed in insect cells.
Molecular Mass
The total predicted molecular weight is 55.4 kD. The non-reduced protein migrates at approximately 60 kD and the DTT-reduced protein produces two bands at approximately 19 kD (Leu 20-Ala 196) and 40 kD (Met 23-Ser 335) by SDS-PAGE.
Purity
Purity is >95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation
For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.
Concentration
10 and 25 µg sizes are bottled at 400 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 ≤1.5 ng/ml, corresponding to a specific activity of ≥0.66 x 106 units/mg, as determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

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Product Citations
  1. Puleston DJ, et al. 2021. Cell. 184:4186. PubMed
  2. Liu W, et al. 2022. Stem Cells Int. 2022:1052166. PubMed
  3. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  4. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  5. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  6. Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed
  7. Ando N, et al. 2015. J Invest Dermatol. . PubMed
  8. Li W, et al. 2019. Front Immunol. 10:833. PubMed
  9. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  10. Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed
  11. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  12. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  13. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  14. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  15. Zhang Y, et al. 2020. Aging (Albany NY). 12:13354. PubMed
  16. Ji J, et al. 2021. Front Immunol. 12:787565. PubMed
  17. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  18. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  19. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  20. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  21. Kaushik DK, et al. 2019. J Clin Invest. 130. PubMed
  22. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  23. Shin B, et al. 2020. Cell Rep. 1898:30. PubMed
  24. Luo X, et al. 2021. Neuron. 109:2691. PubMed
  25. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  26. Li H, et al. 2019. Cancer Res. 79:2167. PubMed
  27. Cho JJ, et al. 2019. Nat Commun. 10:701. PubMed
  28. Le A, et al. 2021. Front Immunol. 11:604785. PubMed
  29. Xu K, et al. 2021. STAR Protoc. 2:100757. PubMed
RRID
AB_10663413 (BioLegend Cat. No. 589002)

Antigen Details

Structure
Heterodimer
Distribution

IL-23 is highly expressed by activated dendritic cells, macrophages, and epidermal Langerhans cells.

Function
IL-23 promotes Th17 responses in vivo, and it is important for the survival and population expansion of Th17 cells. IL-23 induces proliferation of memory T cells and the induction of IL-17A and IL-17F in these cells. IL-23 induces the secretion of IL-17, IL-17F, IL-6, and TNFα in Th17 cells. In conjunction with IL-1, IL-23 is sufficient to induce naïve human T cells to produce IL-17A, IL-17F, IL-22, IL-26, IFN-γ CCL20/MIP-3α and the transcription factor RORγt. PGE2 favors the production of IL-23 and inhibit IL-12 in dendritic cells. PGE2 synergizes with IL-23 in expanding Th17 cells from purified memory CD4+ T cells activated in the absence of accessory cells. IL-23 induces IFN-γ production from T cells treated with PHA. In addition, IL-23 plays a key role in the pathogenesis of autoimmune and chronic inflammatory disorders. This role is supported by the identification of IL-23 receptor (IL-23R) susceptibility alleles associated with inflammatory bowel disease, psoriasis, and ankylosing spondylitis.
Ligand/Receptor
IL-23 binds to L-12Rb1 and IL-23R
Cell Targets
Memory T cells, Th17 cells, Inflammatory macrophages, NK cells, dendritic cells, and monocytes.
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Oppmann B, et al. 2000. Immunity 13:715.
  2. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
  3. Piskin G, et al. 2006. J. Immunol. 176:1908.
  4. Ivanov II, et al. 2006. Cell 126:1121.
  5. McGeachy MJ, et al. 2007. Nat. Immunol. 8:1390.
  6. Wilson NJ, et al. 2007. Nat. Immunol. 8:950.
  7. Bonifac B, et al. 2008. Immunol. Rev. 226:132.
  8. Chizzolini C, et al. 2008. Blood 112:3696.
  9. Buonocore S, et al. 2010. Nature 464:1371.
Gene ID
16160 View all products for this Gene ID 83430 View all products for this Gene ID
UniProt
View information about IL-23 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 8    Revision Date: 10/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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