Loading...

FITC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20_FITC_090507
SJL mouse splenocytes stained with A20 FITC
  • A20_FITC_090507
    SJL mouse splenocytes stained with A20 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
110705 50 µg 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110706 500 µg 88€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  4. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  5. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  6. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  7. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  8. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  9. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  10. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  11. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  12. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  13. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  14. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  15. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  16. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  17. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  18. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  19. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  22. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  23. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  24. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  25. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  26. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  27. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  28. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  29. Chen S, et al. 2022. Cancer Discov. :. PubMed
  30. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  31. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  32. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  33. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  34. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  35. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  36. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  37. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  38. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  39. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  40. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  41. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  42. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  43. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  44. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  45. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  46. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  47. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  48. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  49. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  50. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  51. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  52. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  53. He L, et al. 2019. iScience. 0.790972222. PubMed
  54. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  55. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  56. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  57. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  58. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  59. Ise W, et al. 2018. Immunity. 48:702. PubMed
  60. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  61. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  62. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  63. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  64. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  65. Wan X, et al. 2018. Nature. 560:107. PubMed
  66. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  67. Xu X, et al. 2018. Cell. 175:1336. PubMed
  68. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  69. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  70. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  71. Port JR, et al. 2020. J Virol. 94:. PubMed
  72. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  73. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  74. Jameson K 2009. J Exp Med. 206:2253. PubMed
  75. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  76. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  77. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  78. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  79. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  80. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  81. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  82. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  83. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  84. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  85. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  86. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  87. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  88. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  89. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  90. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  91. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  92. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  93. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  94. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  95. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  96. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  97. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  98. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  99. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  100. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  101. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  102. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  103. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  104. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  105. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  106. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  107. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  108. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  109. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  110. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  111. Young K, et al. 2021. Cell Stem Cell. . PubMed
  112. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  113. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  114. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  115. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313494 (BioLegend Cat. No. 110705)
AB_313494 (BioLegend Cat. No. 110706)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD45.1
Specificity Alt (DOES NOT SHOW ON TDS):
CD45.1
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account