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PerCP/Cyanine5.5 anti-human CD88 (C5aR) Antibody

Pricing & Availability
Clone
S5/1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S5slash1_PerCPCy55_051012
Human peripheral blood granulocytes were stained with CD88 (clone S5/1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • S5slash1_PerCPCy55_051012
    Human peripheral blood granulocytes were stained with CD88 (clone S5/1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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344311 25 tests 141€
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344312 100 tests 306€
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Description

CD88 is a single chain protein with seven membrane-spanning regions and has a MW of 43 kD. CD88 is expressed by monocytes, neutrophils and eosinophils. Also, there have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release and superoxide anion production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone S5/1 blocks the binding of C5a to CD88.

Application References
  1. Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
  2. Elsner J, et al. 1994. Blood 83:3324.
  3. Oppermann M, et al. 1993. J. Immunol. 151:3785.
Product Citations
  1. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  2. França TT, et al. 2021. JCI Insight. 6:. PubMed
  3. Metzemaekers M, et al. 2021. Clin Transl Immunology. 10:e1271. PubMed
RRID
AB_11203904 (BioLegend Cat. No. 344311)
AB_11203904 (BioLegend Cat. No. 344312)

Antigen Details

Structure
CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
Distribution

CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.

Function
After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

Gene ID
728 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD88
Specificity Alt (DOES NOT SHOW ON TDS):
CD88
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD88 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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