- Clone
- L243 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Class II, MHC class II
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
-
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19
Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669). - Additional Product Notes
-
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
-
- Brodsky F. 1984. Immunogenetics 19:179.
- Robbins P, et al. 1987. Human Immunol. 18:301.
- Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
- Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
- Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
- Zipf T, et al. 1981. Cancer Res. 41:4786.
- Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
- Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
- Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
- Wang RF, et al. 1999. Science 284:1351. (Block)
- Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
- Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Stein R, et al. 2011. Leuk. Lymphoma 52:273.
- Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
- Moro M, et al. 2005. BMC Immunol. 6:24.
- Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_493175 (BioLegend Cat. No. 307619)
AB_493175 (BioLegend Cat. No. 307620)
AB_493175 (BioLegend Cat. No. 307656)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
- Distribution
-
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Peptide presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- UniProt
- View information about HLA-DR on UniProt.org
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All HLA-DR Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 APC -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 FITC -
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE -
PE/Cyanine5 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
APC/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... Confocal image of human lymph node sample acquired using the... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... -
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Pacific... -
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
PerCP anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PerCP -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 605™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 421™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 570™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 711™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 785™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 510™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Ultra-LEAF™ Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 650™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human HLA-DR (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 174Yb-an... -
PE/Dazzle™ 594 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 PE... -
Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0159 anti-human HLA-DR
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TotalSeq™-B0159 anti-human HLA-DR
-
TotalSeq™-C0159 anti-human HLA-DR
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Brilliant Violet 750™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Fire™ 640 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 538 anti-human HLA-DR Antibody
Human peripheral blood lymphocytes were stained with CD19 AP... -
KIRAVIA Blue 520™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0159 anti-human HLA-DR
-
PE/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes and ... -
Spark Violet™ 500 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human HLA-DR
Human peripheral blood lymphocytes stained with anti-human C... -
Spark Blue™ 515 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark NIR™ 685 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™)
-
Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™)
-
PE/Fire™ 744 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV395™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 423 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai...
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