Alexa Fluor® 647 anti-human CD163 Recombinant Antibody, CD163, QA19A16

Pricing & Availability

Clone: QA19A16 (See other available formats)
Regulatory Status: RUO
Other Names: GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
Isotype: Mouse IgG1, κ
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Product Citations: publications

QA19A16_Alexa Fluor 647_CD163_Recombinant_Antibody_1_042822 — Human peripheral blood monocytes were stained with anti-human CD163 recombinant (clone QA19A16) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).

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364305

25 tests

90€

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364306

100 tests

244€

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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested

IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2922575 (BioLegend Cat. No. 364305)
AB_2922575 (BioLegend Cat. No. 364306)

Antigen Details

Structure: 134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
Distribution: Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Function: Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor: Haptoglobin-hemoglobin complex, TWEAK
Antigen References: Roth J, et al. 1994. Transolantation. 57:127

Van den Heuvel MM, et al.1999. J Leukoc Biol. 66:858

Sulahian TH, et al. 2000. Cytokines. 12:1312

Fabriek BO, et al. 2007. J Neuroimmunol. 187:179
Gene ID: 9332 View all products for this Gene ID
UniProt: View information about CD163 on UniProt.org

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Other Formats

View All CD163 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD163 Recombinant Antibody	QA19A16	FC,IHC-P,SB
Alexa Fluor® 594 anti-human CD163 Recombinant Antibody	QA19A16	IHC-P
Alexa Fluor® 647 anti-human CD163 Recombinant Antibody	QA19A16	FC,IHC-P
TotalSeq™-Bn1307 anti-human CD163 Recombinant Antibody	QA19A16	SB

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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