- Clone
- 6C3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 6C3/BP-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6C3_Alx647_A_020708 6C3_Alx647_A_020708](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6C3_Alx647_A_020708.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control -
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
108311 | 25 µg | 81€ | ||||
108312 | 100 µg | 184€ |
Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.
- Application References
-
- Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
- Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
- Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
- Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
- Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
- Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
- Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
- Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
- Product Citations
-
- RRID
-
AB_2099614 (BioLegend Cat. No. 108311)
AB_2099613 (BioLegend Cat. No. 108312)
Antigen Details
- Structure
- Aminopeptidase A, 140 kD
- Distribution
-
B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
- Function
- Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
- Cell Type
- B cells, Dendritic cells, Epithelial cells
- Biology Area
- Immunology
- Antigen References
-
1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681. - Gene ID
- 13809 View all products for this Gene ID
- UniProt
- View information about Ly-51 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Ly-51 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse Ly-51 | 6C3 | FC |
FITC anti-mouse Ly-51 | 6C3 | FC |
PE anti-mouse Ly-51 | 6C3 | FC |
Purified anti-mouse Ly-51 | 6C3 | FC,IP,IHC-F |
Alexa Fluor® 647 anti-mouse Ly-51 | 6C3 | FC |
PE/Cyanine7 anti-mouse Ly-51 | 6C3 | FC |
PerCP/Cyanine5.5 anti-mouse Ly-51 | 6C3 | FC |
TotalSeq™-C1034 anti-mouse Ly-51 | 6C3 | PG |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/RMV-7_PE_051311.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-mouse Ly-51
C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220... -
FITC anti-mouse Ly-51
C57BL/6 mouse bone marrow cells were stained with B200 APC a... -
PE anti-mouse Ly-51
C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220... -
Purified anti-mouse Ly-51
C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220... -
Alexa Fluor® 647 anti-mouse Ly-51
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
PE/Cyanine7 anti-mouse Ly-51
C57BL/6 mouse bone marrow cells were stained with CD45R FITC... -
PerCP/Cyanine5.5 anti-mouse Ly-51
C57BL/6 mouse bone marrow lymphocytes were stained with CD45... -
TotalSeq™-C1034 anti-mouse Ly-51
Follow Us