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Alexa Fluor® 700 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Alx700_080408.jpg
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • 6D5_Alx700_080408.jpg
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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115527 25 µg 76€
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115528 100 µg 172€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  2. Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed
  3. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  4. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  5. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  6. Xiao R, et al. 2022. J Immunol. 208:2109. PubMed
  7. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  8. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  9. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  10. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  11. Li J, et al. 2010. J Immunol. 184:6142. PubMed
  12. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  13. Boldison J, et al. 2020. Cell Mol Immunol. 17:843. PubMed
  14. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  15. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  16. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  17. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  18. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  19. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  20. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  21. Perry D, et al. 2012. J Immunol. 189:793. PubMed
  22. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  23. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  24. Roy R, et al. 2022. Elife. 11:. PubMed
  25. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  26. Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
  27. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  28. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  29. Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
  30. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  31. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  32. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  33. Li H, et al. 2020. Nature. 584:274. PubMed
  34. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  35. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  36. Giampazolias E, et al. 2021. Cell. . PubMed
  37. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  38. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  39. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  40. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  41. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  42. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  43. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  44. Bennion BG, et al. 2019. J Virol. 93. PubMed
  45. Rinaldi S, et al. 2021. PLoS Pathog. 17:e1009533. PubMed
  46. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  47. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  48. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  49. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  50. Gorman M, et al. 2016. J Virol. 90: 8212 - 8225. PubMed
  51. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  52. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID
AB_493734 (BioLegend Cat. No. 115527)
AB_493734 (BioLegend Cat. No. 115528)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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