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Alexa Fluor® 700 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
C068C2_A700_CD206_Antibody_1_070616
Thioglycollate-elicited BALB/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b (clone M3/84) PE and CD206 (clone C068C2) Alexa Fluor® 700 (top) or rat IgG2a, κ Alexa Fluor® 700 isotype control (bottom).
  • C068C2_A700_CD206_Antibody_1_070616
    Thioglycollate-elicited BALB/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b (clone M3/84) PE and CD206 (clone C068C2) Alexa Fluor® 700 (top) or rat IgG2a, κ Alexa Fluor® 700 isotype control (bottom).
  • C068C2_A700_CD206_Antibody_2_070616
Compare all formats See Alexa Fluor® 700 spectral data
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141733 25 µg 104€
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141734 100 µg 235€
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xu S, et al. 2022. EBioMedicine. 86:104312. PubMed
  2. Zhang Y, et al. 2022. Front Oncol. 12:1042250. PubMed
  3. Cansever D, et al. 2023. Nat Immunol. . PubMed
  4. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  5. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  6. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  7. Mia MM, et al. 2020. PLoS Biol. 18:e3000941. PubMed
  8. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  9. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  10. Wu X, et al. 2021. Elife. 10:. PubMed
  11. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  12. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  13. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  14. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  15. Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed
  16. Barnette DN, et al. 2018. JCI Insight. 3. PubMed
  17. Kapralov AA, et al. 2020. Nat Chem Biol. 278:16. PubMed
  18. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_2629636 (BioLegend Cat. No. 141733)
AB_2629636 (BioLegend Cat. No. 141734)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 1    Revision Date: 07/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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