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APC anti-mouse CD319 Antibody

Pricing & Availability
Clone
W19300I (See other available formats)
Regulatory Status
RUO
Other Names
SLAMF7, CS1, CRACC, CD319, 19A
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19300I_APC_CD319_Anitbody_051823
C57BL/6 mouse splenocytes were surface stained with anti-mouse CD19 FITC and anti-mouse CD319 (clone W19300I) APC (left) or rat IgG2a, κ APC isotype control (right). Data shown were gated on viable splenocytes.
  • W19300I_APC_CD319_Anitbody_051823
    C57BL/6 mouse splenocytes were surface stained with anti-mouse CD19 FITC and anti-mouse CD319 (clone W19300I) APC (left) or rat IgG2a, κ APC isotype control (right). Data shown were gated on viable splenocytes.
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118805 25 µg 128€
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118806 100 µg 296€
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Description

CD319/CS1/CRACC/SLAMF7 (Signaling lymphocytic activation molecule family member7) is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily. It is expressed on most and low or absent on monocytes and basophils.

CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules. CD319 is a self-ligand and homophilic interaction of CD319 regulates NK cell cytolytic activity. This mouse CD319 antibody does not show cross-reactivity with rat or human.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2941412 (BioLegend Cat. No. 118805)
AB_2941412 (BioLegend Cat. No. 118806)

Antigen Details

Structure
Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
Distribution

Spleen, lymph node, bone marrow, testis, thymus, NK cells, B cells, and subsets of T cells

Function
Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT)
Interaction
Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT)
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Li Y, et al. 2013. PLoS One. 8(9):e76681.
  2. Dongre P, et al. 2013. Mol Immunol. 54:254-63.
  3. Tovar V, et al. 2002. Immunogenetics. 54:394-402.
  4. Dong Z, et al. 2009. Nat Immunol. 10:973-80.
Gene ID
75345 View all products for this Gene ID
UniProt
View information about CD319 on UniProt.org
Go To Top Version: 1    Revision Date: 05/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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