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APC anti-mouse CD66a (CEACAM1a) Antibody

Pricing & Availability
Clone
MAb-CC1 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1a, Bgp
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MAb-CC1_APC_CD66a_Antibody_1_102016
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD66a (clone MAb-CC1) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • MAb-CC1_APC_CD66a_Antibody_1_102016
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD66a (clone MAb-CC1) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • MAb-CC1_APC_CD66a_Antibody_2_102016
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134509 25 µg 110€
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134510 100 µg 271€
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Description

CD66a, known as CEACAM1a, carcinoembryonic antigen-related cell adhesion molecule 1a, is a glycoprotein of the immunoglobulin superfamily and the carcinoembryonic antigen family. Isoforms expressing either two or four alternatively spliced Ig-like domains in mice have been found in a number of epithelial, endothelial, or hematopoietic tissues. CEACAM1a functions as an intercellular adhesion molecule, an angiogenic factor, and a tumor cell growth inhibitor. It also serves as a signal regulatory protein influencing B cell receptor complex-mediated activation. The mouse and human CEACAM1a proteins are targets of viral or bacterial pathogens, respectively. It was reported that targeted disruption of the CEACAM1a gene resulting in a partial ablation of the protein in mice led to reduced susceptibility to virus infection. The antibody recognizes the N-terminal domain of murine CEACAM1a, it does not recognize murine CEACAM1b, an allele in SJL mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Mab-CC1 clone recognizes the MHV binding domain of CD66a2. Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A592. SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells2.

Application References
  1. Turner BC, et al. 2004. J. Virol. 78 (10):5486
  2. Williams RK, et al. 1990. J. Virol. 64:3817
  3. Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716
Product Citations
  1. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  2. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID
AB_2632613 (BioLegend Cat. No. 134509)
AB_2632613 (BioLegend Cat. No. 134510)

Antigen Details

Structure
A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
Distribution

CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.

Function
CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Nakagaki K, et al. 2005. J. Virol. 79(10):6102
2. Greicius G et al. 2003. J. Leukoc. Biol. 74(1):126
3. Hemmila E et al. 2004. J. Virol. 78(18):10156

Gene ID
26365 View all products for this Gene ID
UniProt
View information about CD66a on UniProt.org
Go To Top Version: 2    Revision Date: 01/05/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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