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APC anti-mouse CD88 (C5aR) Antibody

Pricing & Availability
Clone
20/70 (See other available formats)
Regulatory Status
RUO
Other Names
C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
20slash70_APC_1_042211
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C FITC and C5aR (clone 20/70) APC (top) or rat IgG2b, κ APC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • 20slash70_APC_1_042211
    C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C FITC and C5aR (clone 20/70) APC (top) or rat IgG2b, κ APC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • 20slash70_APC_2_042211
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135807 25 µg 91€
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135808 100 µg 214€
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Description

CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mC5aR transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: blocking of C5a binding.

Application References
  1. Godau J, et al. 2004. J. Immunol. 173:3437.
  2. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
  3. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Product Citations
  1. Campisi L, et al. 2022. Nature. 606:945. PubMed
  2. Liu H, et al. 2020. J Immunol. 1954:204. PubMed
  3. Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
  4. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  5. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  6. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  7. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  8. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
RRID
AB_10896758 (BioLegend Cat. No. 135807)
AB_10896758 (BioLegend Cat. No. 135808)

Antigen Details

Structure
Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution

Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.

Function
After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Bioactivity
Chemotaxis, degranulation.
Cell Type
Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

Gene ID
12273 View all products for this Gene ID
UniProt
View information about CD88 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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