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APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_APCCyanine7_CD117_Antibody_022519
C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • 2B8_APCCyanine7_CD117_Antibody_022519
    C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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105825 25 µg 76€
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105826 100 µg 184€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
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  5. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
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  7. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  8. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  9. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  10. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  11. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  12. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  13. Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed
  14. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  15. Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  18. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  19. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  20. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  21. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  22. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  23. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  24. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  25. Iturri L, et al. 2021. Immunity. . PubMed
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  27. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  28. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
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  30. Taylor D, et al. 2011. J Immunol. 186:5956. PubMed
  31. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  32. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  33. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  34. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  35. Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed
  36. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  37. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  38. Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed
  39. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  40. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  41. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  42. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  43. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  44. Reeh K, et al. 2014. Development. 141:2950. PubMed
  45. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
  46. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  47. Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed
  48. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  49. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  50. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  51. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  52. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
  53. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  54. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  55. Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed
  56. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  57. Ding P, et al. 2022. Bone Res. 10:42. PubMed
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  59. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  60. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  61. Cazares O, et al. 2021. Development. 148:. PubMed
  62. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  63. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  64. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  65. Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed
  66. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  67. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  68. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  69. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  70. Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed
  71. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  72. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  73. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  74. Koide S, et al. 2022. iScience. 25:103603. PubMed
  75. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  76. Kassas–Guediri A, et al. 2017. PLoS One. 12:e0173815. PubMed
  77. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  78. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  79. Lee SG, et al. 2020. J Nucl Med. . PubMed
  80. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  81. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  82. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  83. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  84. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  85. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  86. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  87. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
  88. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  89. Chavez JS, et al. 2022. Cells. 11:. PubMed
  90. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  91. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  92. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  93. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  94. Chaudhury S, et al. 2018. Nat Commun. 9:5280. PubMed
  95. Dingler FA, et al. 2020. Mol Cell. . PubMed
  96. Young K, et al. 2021. Cell Stem Cell. . PubMed
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RRID
AB_1626278 (BioLegend Cat. No. 105825)
AB_1626278 (BioLegend Cat. No. 105826)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 3    Revision Date: 02/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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