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APC/Fire™ 750 anti-human CD25 Antibody

Pricing & Availability
Clone
S20019D (See other available formats)
Regulatory Status
RUO
Other Names
interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_S20019D_APCFire750_CD25_Antibody_2_071524
Human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) Pacific Blue™ and anti-human CD25 (clone S20019D) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
  • A_S20019D_APCFire750_CD25_Antibody_2_071524
    Human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) Pacific Blue™ and anti-human CD25 (clone S20019D) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
  • B_S20019D_APCFire750_CD25_Antibody_1_071524
    PHA-activated (3 days) human peripheral blood cells were stained with anti-human CD25 (clone S20019D) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram). Data shown was gated on the lymphocyte population.
Compare all formats See APC/Fire™ 750 spectral data
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385217 25 tests 128€
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385218 100 tests 264€
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Description

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD25 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.

Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.

Clone S20019D does not affect binding of IL-2 to the receptor. 

Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.

RRID
AB_3662282 (BioLegend Cat. No. 385217)
AB_3662282 (BioLegend Cat. No. 385218)

Antigen Details

Structure
55 kD transmembrane protein
Distribution

Activated T cells, T regulatory cells, monocytes

Function
IL-2 receptor
Interaction
CD122, CD132
Ligand/Receptor
IL-2
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Waldmann T. 1991. J Biol Chem. 266:2681-4.
  3. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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