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Biotin anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEC13dot3_Biotin_080607
C57BL/6 mouse splenocytes stained with biotinylated MEC13.3, followed by Sav-PE
  • MEC13dot3_Biotin_080607
    C57BL/6 mouse splenocytes stained with biotinylated MEC13.3, followed by Sav-PE
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102503 50 µg 67€
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102504 500 µg 238€
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Theilmann AL, et al. 2020. Arterioscler Thromb Vasc Biol. 40:2605. PubMed
  2. Mo C, et al. 2022. EMBO J. 41:e108415. PubMed
  3. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
  4. Hao M, et al. 2023. Acta Pharm Sin B. 13:1110. PubMed
  5. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  6. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  7. Corliss BA, et al. 2019. Front Physiol. 1.38125. PubMed
  8. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  9. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  10. Wang C, et al. 2018. Am J Pathol. 189:648. PubMed
  11. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  12. He Q, et al. 2019. Sci Rep. 9:7548. PubMed
  13. Hishikawa A, et al. 2019. Cell Rep. 26:1318. PubMed
  14. De Bruijn HS, et al. 2020. Theranostics. 2.108333333. PubMed
  15. Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed
  16. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  17. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  18. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  19. Goossens EAC, et al. 2021. Int J Mol Sci. 22:. PubMed
  20. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
  21. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
  22. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  23. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
  24. Urner S, et al. 2019. EMBO J. 38:. PubMed
  25. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  26. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  27. Murakami A, et al. 2010. Nephrol Dial Transplant. 25:3884. PubMed
  28. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  29. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  30. Suzuki E, et al. 2012. Clin Immunol. 145:201. PubMed
  31. Laurin M, et al. 2019. Elife. 8. PubMed
  32. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  33. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
RRID
AB_312910 (BioLegend Cat. No. 102503)
AB_312910 (BioLegend Cat. No. 102504)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 07/19/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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