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Brilliant Violet 421™ anti-human CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
MI15 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B-B4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MI15_BV421_CD138_Antibody_FC_110413.jpg
Human myeloma cell line U266 was stained with CD138 (clone MI15) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • MI15_BV421_CD138_Antibody_FC_110413.jpg
    Human myeloma cell line U266 was stained with CD138 (clone MI15) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
356515 25 tests 152€
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356516 100 tests 331€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.4

Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6.

Application References
  1. Costes V, et al. 1999. Hum. Pathol. 30:1405. (IF)
  2. Gattei V, et al. 1999. Br. J. Haematol. 104:152. (WB)
  3. Bologna-Molina R, et al. 2008. Oral Oncol. 44:805. (IHC)
  4. Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482.
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  2. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  3. Lucas C, et al. 2021. Nature. Online ahead of print. PubMed
  4. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  5. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  6. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
RRID
AB_2562659 (BioLegend Cat. No. 356515)
AB_2562659 (BioLegend Cat. No. 356516)

Antigen Details

Structure
100-200 kD type I integral transmembrane glycoprotein
Distribution

Plasma cells, pre-B cells, epithelial cells, endothelial cells

Function
Adhesion, controls cell morphology, regulates cell growth
Ligand/Receptor
FGFb, collagen, fibronectin
Cell Type
B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication.

Gene ID
6382 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD138 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human CD138 (Syndecan-1) MI15 FC,SB
Purified anti-human CD138 (Syndecan-1) MI15 FC,ICC,WB,IHC-P,SB
APC anti-human CD138 (Syndecan-1) MI15 FC
FITC anti-human CD138 (Syndecan-1) MI15 FC,SB
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1) MI15 FC
Alexa Fluor® 700 anti-human CD138 (Syndecan-1) MI15 FC
PE/Cyanine7 anti-human CD138 (Syndecan-1) MI15 FC
Brilliant Violet 421™ anti-human CD138 (Syndecan-1) MI15 FC
Brilliant Violet 510™ anti-human CD138 (Syndecan-1) MI15 FC
Brilliant Violet 605™ anti-human CD138 (Syndecan-1) MI15 FC
Brilliant Violet 711™ anti-human CD138 (Syndecan-1) MI15 FC
Alexa Fluor® 647 anti-human CD138 (Syndecan-1) MI15 FC,SB
Alexa Fluor® 594 anti-human CD138 (Syndecan-1) MI15 ICC
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1) MI15 FC
APC/Cyanine7 anti-human CD138 (Syndecan-1) MI15 FC
Pacific Blue™ anti-human CD138 (Syndecan-1) MI15 FC
TotalSeq™-A0055 anti-human CD138 (Syndecan-1) MI15 PG
Brilliant Violet 785™ anti-human CD138 (Syndecan-1) MI15 FC
Biotin anti-human CD138 (Syndecan-1) MI15 FC
TotalSeq™-C0055 anti-human CD138 (Syndecan-1) MI15 PG
APC/Fire™ 750 anti-human CD138 (Syndecan-1) MI15 FC
TotalSeq™-B0055 anti-human CD138 (Syndecan-1) MI15 PG
PE/Cyanine5 anti-human CD138 (Syndecan-1) MI15 FC
TotalSeq™-D0055 anti-human CD138 (Syndecan-1) MI15 PG
PE/Fire™ 640 anti-human CD138 (Syndecan-1) MI15 FC
Spark Violet™ 500 anti-human CD138 (Syndecan-1) MI15 FC
FITC anti-human CD138 MI15 FC
PE/Fire™ 700 anti-human CD138 (Syndecan-1) MI15 FC
Spark Violet™ 423 anti-human CD138 (Syndecan-1) MI15 FC
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™) MI15 FC
GMP FITC anti-human CD138 (Syndecan-1) MI15 FC
Brilliant Violet 650™ anti-human CD138 (Syndecan-1) MI15 FC
Go To Top Version: 2    Revision Date: 06/09/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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