- Clone
- A2F10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Flk-2, Flt3, Ly-72
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Flt3 transfected cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of the this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application References
-
- Sergejeva S, et al. 2004. Blood 103:1270.
- Auffray C, et al. 2009. J. Exp. Med. 206:595.
- Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
- Product Citations
-
- RRID
-
AB_2562338 (BioLegend Cat. No. 135313)
AB_2562338 (BioLegend Cat. No. 135315)
AB_2562338 (BioLegend Cat. No. 135314)
Antigen Details
- Structure
- A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
- Distribution
-
Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
- Function
- Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
- Ligand/Receptor
- Flk-2/FLT3 ligand
- Cell Type
- B cells, Hematopoietic stem and progenitors
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478 - Gene ID
- 14255 View all products for this Gene ID
- UniProt
- View information about CD135 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD135 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD135 | A2F10 | FC |
Biotin anti-mouse CD135 | A2F10 | FC |
APC anti-mouse CD135 | A2F10 | FC |
PE/Cyanine5 anti-mouse CD135 | A2F10 | FC |
Brilliant Violet 421™ anti-mouse CD135 | A2F10 | FC |
TotalSeq™-A0098 anti-mouse CD135 | A2F10 | PG |
TotalSeq™-B0098 anti-mouse CD135 | A2F10 | PG |
TotalSeq™-C0098 anti-mouse CD135 | A2F10 | PG |
KIRAVIA Blue 520™ anti-mouse CD135 | A2F10 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-mouse CD135
Bone marrow cells from C57BL/6 mouse stained with A2F10 PE a... Bone marrow cells from C57BL/6 mouse stained with rat IgG2a,... -
Biotin anti-mouse CD135
C57BL/6 bone marrow cells stained with CD45R/B220 + CD11b AP... -
APC anti-mouse CD135
C57BL/6 bone marrow cells stained with A2F10 APC and CD45R/B... C57BL/6 bone marrow cells stained with rat IgG2a APC isotype... -
PE/Cyanine5 anti-mouse CD135
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Brilliant Violet 421™ anti-mouse CD135
C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11... -
TotalSeq™-A0098 anti-mouse CD135
-
TotalSeq™-B0098 anti-mouse CD135
-
TotalSeq™-C0098 anti-mouse CD135
-
KIRAVIA Blue 520™ anti-mouse CD135
C57BL/6 bone marrow cells stained with anti-mouse CD45R/B220...
Follow Us