Brilliant Violet 510 anti-human CD56 NCAM Antibody anti-CD56 (NCAM)

Pricing & Availability

Clone: 5.1H11 (See other available formats)
Regulatory Status: RUO
Other Names: Leu-19, NKH1, NCAM-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

5.1H11_BV510_CD56_Antibody_FC_042915 — Human peripheral blood lymphocytes were stained with CD56 (clone 5.1H11) Brilliant Violet™ 510 (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).

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362533

25 tests

155€

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362534

100 tests

331€

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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2565632 (BioLegend Cat. No. 362533)
AB_2565632 (BioLegend Cat. No. 362534)

Antigen Details

Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71
3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684 View all products for this Gene ID
UniProt: View information about CD56 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD56 (NCAM) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD56 (NCAM)	5.1H11	FC
APC anti-human CD56 (NCAM)	5.1H11	FC
PerCP/Cyanine5.5 anti-human CD56 (NCAM)	5.1H11	FC
PE anti-human CD56 (NCAM)	5.1H11	FC
PE/Cyanine7 anti-human CD56 (NCAM)	5.1H11	FC
APC/Cyanine7 anti-human CD56 (NCAM)	5.1H11	FC
PE/Cyanine5 anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 647 anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 488 anti-human CD56 (NCAM)	5.1H11	FC
Pacific Blue™ anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 700 anti-human CD56 (NCAM)	5.1H11	FC
PerCP anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 650™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 510™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 421™ anti-human CD56 (NCAM)	5.1H11	FC
Biotin anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 605™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 570™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 711™ anti-human CD56 (NCAM)	5.1H11	FC
PE/Dazzle™ 594 anti-human CD56 (NCAM)	5.1H11	FC
FITC anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 785™ anti-human CD56 (NCAM)	5.1H11	FC
Ultra-LEAF™ Purified anti-human CD56 (NCAM)	5.1H11	FC
APC/Fire™ 750 anti-human CD56 (NCAM)	5.1H11	FC
PE anti-human CD56 (NCAM)	5.1H11	FC
APC anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 750™ anti-human CD56 (NCAM)	5.1H11	FC
TotalSeq™-A0047 anti-human CD56 (NCAM)	5.1H11	PG
TotalSeq™-C0047 anti-human CD56 (NCAM)	5.1H11	PG
TotalSeq™-B0047 anti-human CD56 (NCAM)	5.1H11	PG
Spark NIR™ 685 anti-human CD56 (NCAM)	5.1H11	FC
KIRAVIA Blue 520™ anti-human CD56 (NCAM)	5.1H11	FC
GMP PE anti-human CD56 (NCAM)	5.1H11	FC
TotalSeq™-D0047 anti-human CD56 (NCAM)	5.1H11	PG
GMP APC anti-human CD56 (NCAM)	5.1H11	FC
Spark YG™ 593 anti-human CD56 (NCAM)	5.1H11	FC
Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD56 (NCAM) SF	5.1H11	FC
Spark Red™ 718 anti-human CD56 (NCAM)	5.1H11	FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF	5.1H11	FC
APC/Fire™ 810 anti-human CD56 (NCAM) Antibody	5.1H11	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/30/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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