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Brilliant Violet 605™ anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK1.5_BV605_CD4_Antibody_FC_1_121114
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD4 (clone GK1.5) Brilliant Violet 605™ (top) or rat IgG2b, κ Brilliant Violet 605™ isotype control (bottom).
  • GK1.5_BV605_CD4_Antibody_FC_1_121114
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD4 (clone GK1.5) Brilliant Violet 605™ (top) or rat IgG2b, κ Brilliant Violet 605™ isotype control (bottom).
  • GK1.5_BV605_CD4_Antibody_FC_2_121114
Compare all formats See Brilliant Violet 605™ spectral data
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100451 50 µg 248€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  4. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  5. Yang J, et al. 2022. Mol Ther Nucleic Acids. 30:184. PubMed
  6. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  7. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  8. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  9. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  10. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  11. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  12. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  13. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  14. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  15. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  16. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  17. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  18. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  19. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  20. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  21. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  22. Biancur DE, et al. 2020. Cell Metabolism. 33(1):199-210.e8. PubMed
  23. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  24. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  25. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  26. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  27. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  28. Liu CY, et al. 2020. Cell Rep. 33:108275. PubMed
  29. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  30. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  31. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  32. Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
  33. Shane HL, et al. 2020. Food Chem Toxicol. 136:111114. PubMed
  34. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  35. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  36. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  37. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  38. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  39. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  40. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  41. Vazquez-Lombardi R, et al. 2017. Nat Commun. 8:15373. PubMed
  42. Rouse MD, et al. 2020. Front Microbiol. 11:414. PubMed
  43. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  44. Zhu G, et al. 2022. Front Microbiol. 12:806902. PubMed
  45. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  46. Eckert EC, et al. 2020. Mol Ther Oncolytics. 0.710416667. PubMed
  47. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  48. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  49. Rappaport AR, et al. 2022. Nat Commun. 13:3289. PubMed
  50. Goc J, et al. 2021. Cell. 184:5015. PubMed
  51. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_2564591 (BioLegend Cat. No. 100451)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC,IHC-F,ICC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,ICC,SB
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
Go To Top Version: 1    Revision Date: 12/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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