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Brilliant Violet 650™ anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
104D2 (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
104D2_BV650_CD117_Antibody_FC_102813
Human erythroleukemia cell line (HEL) was stained with CD117 (clone 104D2) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
  • 104D2_BV650_CD117_Antibody_FC_102813
    Human erythroleukemia cell line (HEL) was stained with CD117 (clone 104D2) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
Compare all formats See Brilliant Violet 650™ spectral data
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313221 25 tests 172€
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313222 100 tests 317€
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MOLM-1 megakaryocytic cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.

Application References
  1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  2. Hernandez JD, et al. 2020. Sci Rep. 10:13210. PubMed
  3. Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed
  4. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  5. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
RRID
AB_2562714 (BioLegend Cat. No. 313221)
AB_2562714 (BioLegend Cat. No. 313222)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Modification
Multiple phosphorylation sites
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD117 (c-kit) 104D2 FC,CyTOF®,ICC,IP
PE anti-human CD117 (c-kit) 104D2 FC,SB
APC anti-human CD117 (c-kit) 104D2 FC
Biotin anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine5 anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 421™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 605™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 510™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 650™ anti-human CD117 (c-kit) 104D2 FC
Purified anti-human CD117 (c-kit) (Maxpar® Ready) 104D2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
APC/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 711™ anti-human CD117 (c-kit) 104D2 FC
FITC anti-human CD117 (c-kit) 104D2 FC
Alexa Fluor® 488 anti-human CD117 (c-kit) 104D2 FC,SB
Alexa Fluor® 647 anti-human CD117 (c-kit) 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 785™ anti-human CD117 (c-kit) 104D2 FC
PE anti-human CD117 (c-kit) 104D2 FC
APC anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-A0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-C0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-B0061 anti-human CD117 (c-kit) 104D2 PG
Alexa Fluor® 700 anti-human CD117 (c-kit) 104D2 FC
Spark NIR™ 685 anti-human CD117 (c-kit) Antibody 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-D0061 anti-human CD117 (c-kit) 104D2 PG
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
GMP APC anti-human CD117 (c-kit) 104D2 FC
GMP PE anti-human CD117 (c-kit) 104D2 FC
Spark Red™ 718 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
Go To Top Version: 1    Revision Date: 11/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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