Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 448404 | 5 plates | 299€ | ||||
PLAUR (uPAR), also known as CD87, is a urokinase-type plasminogen activator system consisting of proteinase, receptor, and inhibitor. PLAUR is involved
in proteolysis, cell migration and tissue remodeling by proteolysis, signal transduction and chemokine-like activities. It predominately plays roles in cell activation, adhesion, migration, and extravasation in blood cells.
High serum levels of soluble PLAUR (uPAR) are associated with lower survival rates in cancer and HIV-1 patients. Research on COVID-19 suggests that levels of PLAUR (uPAR) are increased in COVID-19 patients that are hospitalized and are in critical condition.
The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The mouse monoclonal capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and Human PLAUR (uPAR) protein binds to the immobilized capture antibody. Next, biotinylated anti-human PLAUR (uPAR) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of Human PLAUR (uPAR) present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
Kit Contents
- Kit Contents
-
- Human PLAUR (uPAR) ELISA MAX™ Capture Antibody (200X)
- Human PLAUR (uPAR) ELISA MAX™ Detection Antibody (200X)
- Human PLAUR (uPAR) Standard
- Avidin-HRP (1000X)
- Substrate Solution D
- Coating Buffer B (5X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.57 ± 0.43 pg/mL
- Standard Range
- 3.9 – 250 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Molecular Family
- Soluble Receptors
- Gene ID
- NA
- UniProt
- View information about PLAUR on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
Follow Us