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FITC anti-human CD158 (KIR2DL1/S1/S3/S5) Antibody

Pricing & Availability
Clone
HP-MA4 (See other available formats)
Regulatory Status
RUO
Other Names
CD158a, CD158g, CD158h, KIR2DL1, KIR2DS1, KIR2DS3, KIR2DS5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
HP-MA4_FITC_021309.jpg
Human peripheral blood lymphocytes stained with HP-MA4 FITC and CD56 APC
  • HP-MA4_FITC_021309.jpg
    Human peripheral blood lymphocytes stained with HP-MA4 FITC and CD56 APC
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339503 25 tests 81€
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339504 100 tests 184€
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and posses inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. 14 polymorphic KIR genes have been reported in humans. CD158 is mainly expressed on a subset of NK cells and a small population of CD8+ T cells. HLA-C is the ligand of CD158a/h.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell clone LB2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

mAb HP-MA4 reacts with KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).

Additional reported applications include: inhibits NK cell mediated cytotoxicity and immunoprecipitation.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References
  1. De Miguel M and M. Lopez-Botet. 2002. Inmunologia. 21:187
  2. Goodridge JP, et al. 2013. J. Immunol. 191:3553. PubMed
Product Citations
  1. Kroll KW, et al. 2022. Cell Rep Med. 3:100773. PubMed
  2. Hydes T, et al. 2017. Immun Inflamm Dis. 10.1002/iid3.190. PubMed
  3. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  4. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
  5. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  6. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  7. Lim D, et al. 2014. J Immunol. 193:5065. PubMed
  8. Reches A, et al. 2016. J Immunol. 196: 4967 - 4976. PubMed
  9. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  10. Goodridge J, et al. 2013. J Immunol. 191:3553. PubMed
  11. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID
AB_2130378 (BioLegend Cat. No. 339503)
AB_2130378 (BioLegend Cat. No. 339504)

Antigen Details

Structure
Contains two Ig-like extracellular domains, 58/50 kD
Distribution

Subset of NK cells and a small subset of CD8+ T cells

Function
Inhibits NK cell function, play a role in self-tolerance.
Ligand/Receptor
HLA-C
Cell Type
NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication

Gene ID
3802 View all products for this Gene ID 3810 View all products for this Gene ID 3808 View all products for this Gene ID 3806 View all products for this Gene ID
UniProt
View information about CD158 on UniProt.org
Go To Top Version: 2    Revision Date: 12/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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