- Clone
- 4F5/CD32 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
398407 | 25 tests | 72€ | ||||
398408 | 100 tests | 192€ |
CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Extracellular domain of human CD32B
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.
- Application References
-
- Su K, et al. 2007. J Immunol. 178:3272
- RRID
-
AB_2941583 (BioLegend Cat. No. 398407)
AB_2941583 (BioLegend Cat. No. 398408)
Antigen Details
- Structure
- Type I transmembrane protein, 40 kDa, member of the Ig superfamily
- Distribution
-
B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.
- Function
- Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
- Ligand/Receptor
- Fc region of complex or aggregated immunoglobulins gamma
- Cell Type
- B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Bruhns P, et al. 2009. Blood. 113: 3716.
- Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735.
- Descours B, et al. 2017. Nature 543: 564.
- Tomiyama Y, et al. 1992. Blood. 80: 2261.
- Indik Z, et al. 1991. J Clin Invest. 88: 1766.
- Ramsland PA, et al. 2011. J Immunol. 187: 3208.
- Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311.
- Bournazos S, et al. 2009. J Immunol. 182: 8026.
- Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437.
- Sandilands GP, et al. 1997. Immunology. 91: 204.
- Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878.
- Gillis C, et al. 2014. Front Immunol. 5: 254.
- Gene ID
- 2213 View all products for this Gene ID 9103 View all products for this Gene ID
- UniProt
- View information about CD32B CD32C on UniProt.org
Other Formats
View All CD32B/C Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD32B/C | 4F5/CD32 | FC |
PE anti-human CD32B/C Antibody | 4F5/CD32 | FC |
APC anti-human CD32B/C | 4F5/CD32 | FC |
FITC anti-human CD32B/C | 4F5/CD32 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD32B/C
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD32B/C Antibody
Human peripheral blood mononuclear cells were stained with a... -
APC anti-human CD32B/C
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD32B/C
Human peripheral blood lymphocytes were surface stained with...
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