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FITC anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
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Product Citations
publications
GL-1_FITC_090707
LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 FITC
  • GL-1_FITC_090707
    LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 FITC
Compare all formats See FITC spectral data
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105005 50 µg 76€
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105006 500 µg 224€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed
  2. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
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  5. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  6. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  7. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
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  12. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  13. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
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  16. Zhang J, et al. 2021. Front Cardiovasc Med. 8:737652. PubMed
  17. Wang S, et al. 2022. Front Immunol. 13:869061. PubMed
  18. Duan M, et al. 2022. Cell Commun Signal. 20:60. PubMed
  19. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  20. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  21. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  22. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  23. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  24. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  25. Yan H, et al. 2022. J Nanobiotechnology. 20:280. PubMed
  26. Cho H, et al. 2022. Nat Commun. 13:5974. PubMed
  27. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  28. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  29. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  30. Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed
  31. Chen X, et al. 2022. J Appl Oral Sci. 30:e20220316. PubMed
  32. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  33. An W et al. 2019. British journal of pharmacology. 176(5):699-710 . PubMed
  34. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  35. Tsang J, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  36. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  37. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  38. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  39. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  40. Matundan H, et al. 2019. J Virol. 93. PubMed
  41. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  42. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  43. Vogel A, et al. 2022. STAR Protoc. 3:101653. PubMed
  44. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  45. Xiao Y, et al. 2022. Nat Commun. 13:758. PubMed
  46. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  47. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  48. Feng S, et al. 2021. Exp Ther Med. 21:20. PubMed
  49. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  50. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  51. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  52. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  53. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  54. Moon Y, et al. 2022. Theranostics. 12:1999. PubMed
  55. Lin R, et al. 2022. Cell Death Dis. 13:345. PubMed
  56. Smith LK, et al. 2021. Elife. 10:. PubMed
  57. Xue YL, et al. 2020. J Cell Mol Med. 24:12341. PubMed
  58. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  59. Song HY, et al. 2021. Mol Ther. S1525-0016:00472-X. PubMed
  60. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  61. Xu GY, et al. 2022. Small. 18:e2107838. PubMed
  62. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  63. Li C, et al. 2022. Front Cell Dev Biol. 10:913824. PubMed
  64. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  65. Fontana C, et al. 2016. J Biol Chem. 291: 7727-7741. PubMed
  66. Aglas L, et al. 2018. J Allergy Clin Immunol. 142:984. PubMed
  67. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_313148 (BioLegend Cat. No. 105005)
AB_313148 (BioLegend Cat. No. 105006)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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