Loading...

Intracellular Staining Permeabilization Wash Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Perm/Wash, Permeabilization Buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
421002 100 mL 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Intracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining Permeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
The Intracellular Staining Perm wash buffer solution should be stored between 2°C and 8°C. Do not freeze.
Application

ICC, ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

For use in permeabilization, dilute Intracellular Staining Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Intracellular Staining Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the process twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" on BioLegend's website for more details.

Application Notes

The Intracellular Staining Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is heavy precipitation observed after dilution to 1X working solution, the buffer can be filtered to clarify the solution.

Application References
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  3. del Rio ML, et al. 2011. Transplantation. 92:1085. PubMed
  4. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  7. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  8. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
Product Citations
  1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  2. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  3. Kim S, et al. 2020. Development. 147:00:00. PubMed
  4. Dumauthioz N, et al. 2020. Cell Mol Immunol. . PubMed
  5. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  6. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  7. Arbues A, et al. 2020. PLoS Pathog. 16:e1008312. PubMed
  8. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  9. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  10. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  11. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  12. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  13. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  14. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  15. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  16. Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed
  17. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  18. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  19. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  20. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  21. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  22. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  23. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  24. Chan L, et al. 2022. Cells. 12: . PubMed
  25. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  26. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  27. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  28. Di Benedetto C, et al. 2023. Cancers (Basel). 15: . PubMed
  29. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  30. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  31. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  32. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  33. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  34. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  35. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  36. Chen R, et al. 2022. J Mol Cell Biol. 14:. PubMed
  37. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  38. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  39. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  40. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  41. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  42. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  43. Stokes JV, et al. 2022. Curr Protoc. 2:e584. PubMed
  44. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  45. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  46. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  47. Mohamed GA, et al. 2023. Breast Cancer Res. 25:23. PubMed
  48. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  49. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  50. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  51. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  52. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  53. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  54. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  55. Komoto S, et al. 2023. Cancers (Basel). 15:. PubMed
  56. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  57. Glaser K, et al. 2015. Immunol Lett. 166: 19-27. PubMed
  58. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  59. Khayrullina G, et al. 2020. Skelet Muscle. 10:16. PubMed
  60. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  61. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  62. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  63. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  64. Lebedeva IV, et al. 2021. Cell Death Dis. 12:770. PubMed
  65. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  66. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  67. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  68. Stewart CA, et al. 2020. bioRxiv. . PubMed
  69. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  70. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  71. Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed
  72. Stewart CA, et al. 2021. J Thorac Oncol. Online ahead of prin. PubMed
  73. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  74. Poli V, et al. 2021. iScience. 24:103256. PubMed
  75. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  76. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  77. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  78. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  79. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  80. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  81. Goyal S, et al. 2017. Sci Rep. . 10.1038/s41598-017-18046-x. PubMed
  82. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  83. Gärtner F, et al. 2021. Front Immunol. 12:745132. PubMed
  84. Gao F, et al. 2021. Front Cell Infect Microbiol. 10:593805. PubMed
  85. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  86. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  87. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  88. Shinde MY, et al. 2017. J Biol Chem. 292:18240. PubMed
  89. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  90. Ghose J, et al. 2018. Oncoimmunology. 7:e1486948. PubMed
  91. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  92. Lau H, et al. 2021. Int J Mol Sci. 22:. PubMed
  93. Zhou H, et al. 2022. J Clin Invest. 132:. PubMed
  94. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  95. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  96. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  97. Martinez–Usatorre A, et al. 2018. J Immunol. 201:792. PubMed
  98. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  99. Lau H, et al. 2021. Xenotransplantation. 28:e12667. PubMed
  100. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  101. Ma Q, et al. 2022. Nat Commun. 13:4142. PubMed
  102. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  103. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  104. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  105. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  106. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  107. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  108. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  109. Clancy–Thompson E, et al. 2018. Cancer Immunol Res. 1.308333333. PubMed
  110. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  111. Zhang X, et al. 2022. Theranostics. 12:1621. PubMed
  112. Berical A, et al. 2022. Nat Commun. 13:4270. PubMed
  113. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  114. Baek J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.011. PubMed
  115. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  116. Rodriguez S, et al. 2020. Xenotransplantation. 27:e12554. PubMed
  117. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  118. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  119. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  120. Lownik JC, et al. 2019. J Immunol. 202:664. PubMed
  121. Lau H, et al. 2020. Xenotransplantation. 27:e12555. PubMed
  122. Mkhikian H, et al. 2022. Nat Aging. 2:231. PubMed
  123. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  124. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  125. Nausch N, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005415. PubMed
  126. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  127. Breznik JA, et al. 2018. Physiol Rep. 6:e13937. PubMed
  128. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  129. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  130. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  131. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  132. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  133. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  134. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  135. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  136. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  137. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  138. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  139. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  140. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  141. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  142. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  143. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  144. Hayes D, et al. 2022. JCI Insight. 7:. PubMed
  145. Gong C, et al. 2021. Mol Cell. 81:4059. PubMed
  146. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  147. Kim S, et al. 2020. Development. . PubMed
  148. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  149. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  150. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  151. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  152. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  153. Ma K, et al. 2022. iScience. 25:104347. PubMed
  154. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  155. Lau H, et al. 2020. Islets. 41:12. PubMed
  156. Lau H, et al. 2020. PLoS One. 15:e0243506. PubMed
  157. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  158. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  159. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  160. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  161. Haswell JR, et al. 2021. PLoS One. 16:e0252848. PubMed
  162. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  163. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  164. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  165. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  166. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  167. Spiliopoulou P, et al. 2022. Mol Cancer Ther. . PubMed
  168. Tintos-Hernandez JA, et al. 2021. Brain Commun. 3:fcab215. PubMed
  169. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  170. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  171. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  172. Gao J, et al. 2022. Front Immunol. 13:955812. PubMed
  173. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  174. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  175. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  176. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  177. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  178. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  179. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  180. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  181. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  182. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  183. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  184. Lee G, et al. 2021. Viruses. 13: . PubMed
  185. Hernandez AA, et al. 2020. J Immunol. 205:2806. PubMed
  186. Su Y, et al. 2020. Cell. 1479:183. PubMed
  187. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  188. Mcgaw C, et al. 2022. Nat Commun. 13:2833. PubMed
  189. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  190. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  191. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  192. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  193. Giadone RM, et al. 2018. Amyloid. 25:148. PubMed
  194. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  195. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  196. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  197. Wang X, et al. 2022. Elife. 11:. PubMed
  198. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  199. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  200. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  201. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  202. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  203. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  204. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  205. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  206. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  207. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  208. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  209. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/26/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account