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LEGEND MAX™ Mouse IL-22 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-22 Pre-coated ELISA Kit
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Product Citations
publications
mouse il-22_021810
  • mouse il-22_021810
  • mouse il-22_082718
    Naïve CD4 T cells were harvested from the spleens of Il22+/+ or Il22-/- mice (C57BL/6 background, see Immunity. 2007 Oct; 27(4):647-59).

    Cells were then cultured with plate-bound aCD3 and aCD28 mAbs in IMDM (Th0 Conditions); or additionally with recombinant mouse IL-23 (50 ng/ml), IL-1b (20 ng/ml), IL-6 (20 ng/ml) and 200 nM 6-formylindolo(3,2-b)carbazole (Th22 Conditions).

    Three days post-stimulation, supernatants were harvested, and IL-22 was quantitated using the LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307).

    Data were provided courtesy of Prof. Lauren A. Zenewicz, University of Oklahoma.
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436307 1 Pre-coated Plate 328€
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Description

IL-22 belongs to a family of cytokines structurally related to IL-10, including IL-19, IL-20, IL-24, and IL-26. Mouse IL-22 shares 79% amino acid identity with human IL-22. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of the T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in the disregulated inflammation observed in autoimmune diseases.

BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-22 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-22 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Mouse IL-22 Pre-coated 96-well Strip Microplate
  • Mouse IL-22 Dectection Antibody
  • Mouse IL-22 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA
Product Citations
  1. Mukherjee P, et al. 2022. J Lipid Res. 63:100153. PubMed
  2. Ricardo-Gonzalez RR, et al. 2022. Immunity. :. PubMed
  3. Li Y, et al. 2018. Cell Mol Immunol. 15:518. PubMed
  4. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
Sensitivity
1.6 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Th17, Th1, Th2, Th22, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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