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PE anti-human CD154 Antibody

Pricing & Availability
Clone
W20199I (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, TNFSF5, T-BAM, gp39
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20199I_PE_CD154_Antibody_080323
Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423302). Cells were surface stained with anti-human CD154 (clone W20199I) PE (left) or rat IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD4 APC.
  • W20199I_PE_CD154_Antibody_080323
    Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423302). Cells were surface stained with anti-human CD154 (clone W20199I) PE (left) or rat IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD4 APC.
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388203 25 tests 100€
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388204 100 tests 204€
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Description

CD154, also known as CD40L, TNFSF5, T-BAM, and gp39, is a 32-39 kD type II transmembrane glycoprotein member of the TNF superfamily expressed on activated T cells. CD154 interacts as a costimulatory molecule with CD40 on B Cells to induce B cell activation, resulting in proliferation, immunoglobulin production, and Ig Isotype switching. CD40L also may regulate T cell cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD40L protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W20199I is able to block the binding of clone 24-31 on target cells.

Clone W20199I is able to block the binding of CD40 and CD40L interaction on target cells.

RRID
AB_3105982 (BioLegend Cat. No. 388203)
AB_3105982 (BioLegend Cat. No. 388204)

Antigen Details

Structure
32-39 kD type II transmembrane glycoprotein of the TNF superfamily
Distribution

Activated T cells

Function
B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. van Kooten C and Banchereau J. 2000. J Leukoc Biol. 67:2-17.
  2. Ford GS, et al. 1999. J Immunol. 162:4037-44.
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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