- Clone
- 5D3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- ABCG2, CDw338, Bcrp1, MXR
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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332007 | 25 tests | 108€ | ||||
332008 | 100 tests | 234€ |
CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34. In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. The expression of ABCG2 appears to be highly conserved as it has been identified in various species.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This clone is listed under the following US Patent Numbers:
6,313,277; 7,541,437; 7,655,755; 7,741,455 - Application References
- Product Citations
-
- RRID
-
AB_2220319 (BioLegend Cat. No. 332007)
AB_2220319 (BioLegend Cat. No. 332008)
Antigen Details
- Distribution
-
primitive 'sidepopulation' (SP) stem cells, KDR+ human stem cells
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kim M, et al. 2002. Clin. Cancer Res. 8:228.
2. Scharenber CW, et al. 2002. Blood 99:50712.
3.Goodell MA, et al. 1997. Nat. Med. 3:133745.
4. Bunting KD. 2002. Stem Cells. 20:11. - Gene ID
- 9429 View all products for this Gene ID
- UniProt
- View information about CD338 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD338 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD338 (ABCG2) | 5D3 | FC |
FITC anti-human CD338 (ABCG2) | 5D3 | FC |
APC anti-human CD338 (ABCG2) | 5D3 | FC |
PerCP/Cyanine5.5 anti-human CD338 (ABCG2) | 5D3 | FC |
PE/Cyanine7 anti-human CD338 (ABCG2) | 5D3 | FC |
TotalSeq™-C0569 anti-human CD338 (ABCG2) | 5D3 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 PE -
FITC anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with 5D3 FITC -
APC anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 APC -
PerCP/Cyanine5.5 anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with CD338 (ABCG2) (clone 5D... -
PE/Cyanine7 anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with CD338 (ABCG2) (clone 5D... -
TotalSeq™-C0569 anti-human CD338 (ABCG2)
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