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PE anti-human CD178 (FasL) Antibody

Pricing & Availability
Clone
W19402J (See other available formats)
Regulatory Status
RUO
Other Names
Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
Isotype
Rat IgG2b, κ
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Product Citations
publications
W19402J_PE_FasL_Antibody_1_092624
Human FAS ligand transfected cells were stained with anti-human CD178 (FasL) (clone W19402J) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • W19402J_PE_FasL_Antibody_1_092624
    Human FAS ligand transfected cells were stained with anti-human CD178 (FasL) (clone W19402J) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
See PE spectral data
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387903 25 tests 88€
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387904 100 tests 216€
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Description

CD178, also known as Fas ligand and CD95L, is a 38-42 kD type II glycoprotein. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human FasL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Distribution

Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells

Function
Apoptosis/Cell Death
Interaction
The interaction of Fas Ligand and FasR on an adjacent cell leads to the trimerization and formation of the death-inducing signaling complex (DISC).
Ligand/Receptor
FASR (CD95)
Cell Type
Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References
  1. Suda T, et al. 1997. J Exp Med. 186:2045-50.
  2. Kayagaki N, et al. 1995. J Exp Med. 182:1777-83.
  3. Tanaka M, et al. 1995. EMBO J. 14:1129-35.
Gene ID
356 View all products for this Gene ID
UniProt
View information about FasL on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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