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PE anti-mouse CD123 Antibody

Pricing & Availability
Clone
5B11 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3 Receptor α chain, IL-3Rα
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5B11_080505.jpg
C57BL/6 mouse bone marrow cells stained with 5B11 PE and CD11b FITC
  • 5B11_080505.jpg
    C57BL/6 mouse bone marrow cells stained with 5B11 PE and CD11b FITC
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106005 25 µg 69€
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Description

CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CTLL cells transfected with the mouse IL-3Rα chain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.

Application References
  1. Ichihara, et al. 1995. EMBO J. 14:939. (FC)
  2. Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC)
  3. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)
Product Citations
  1. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  2. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  3. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
RRID
AB_2124403 (BioLegend Cat. No. 106005)

Antigen Details

Structure
Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD
Distribution

Hematopoietic progenitors, basophils, mast cells, megakaryocytes

Function
Proliferation, differentiation of hematopoietic cells
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Miyajima A, et al. 1993. Blood 82:1960.
3. Itoh N, et al. 1990. Science 247:324.

Gene ID
16188 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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