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PE anti-mouse CD45RB Antibody

Pricing & Availability
Clone
C363-16A (See other available formats)
Regulatory Status
RUO
Other Names
CD45RB
Isotype
Rat IgG2a, κ
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Product Citations
publications
C363-16a
C57BL/6 splenocytes stained with C363-16A PE
  • C363-16a
    C57BL/6 splenocytes stained with C363-16A PE
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103307 50 µg 59€
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103308 200 µg 176€
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Description

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cloned mouse Th2 cell lines
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References
  1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Mishra HK, et al. 2015. J Leukoc Biol. 447:97. PubMed
  2. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  3. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  4. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  5. Shao B, et al. 2019. J Immunol. 10.4049/jimmunol.1900680. PubMed
  6. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  7. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  8. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  9. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  10. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  11. Hao C, et al. 2021. Frontiers in Cell and Developmental Biology. 9:695015. PubMed
  12. Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
  13. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  14. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
RRID
AB_313014 (BioLegend Cat. No. 103307)
AB_313014 (BioLegend Cat. No. 103308)

Antigen Details

Structure
Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution

Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells

Function
Phosphatase, T cell development and activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, Thy1
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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