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PE anti-mouse IL-33Rα (ST2) Antibody

Pricing & Availability
Clone
DIH4 (See other available formats)
Regulatory Status
RUO
Other Names
ST2, ST2L, IL-1R1
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
DIH4_PE_IL-33Ra_Antibody_012618
Mouse Th2 clone D10.G4.1 was stained with PE anti-mouse IL-33Ra (clone DIH4, filled histogram) or PE rat IgG1, κ isotype control (open histogram).
  • DIH4_PE_IL-33Ra_Antibody_012618
    Mouse Th2 clone D10.G4.1 was stained with PE anti-mouse IL-33Ra (clone DIH4, filled histogram) or PE rat IgG1, κ isotype control (open histogram).
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146607 25 µg 113€
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146608 100 µg 288€
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Description

IL-33Ra, also known as ST2 or IL-1RL1 is a member of the Toll/IL-1 receptor family. It binds IL-33 and is structurally similar to IL-1R1. Two forms of the protein exist – soluble form known as ST2 and membrane anchored form known as ST2L. The membrane form is expressed by Th2 cells and bone marrow derived mast cells whereas the soluble form is expressed by serum-stimulated fibroblasts.

Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation. The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies and cardiac disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-33Rα-hFcγ 1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC, Block) PubMed
Product Citations
  1. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  2. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  3. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  4. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_2728176 (BioLegend Cat. No. 146607)
AB_2728176 (BioLegend Cat. No. 146608)

Antigen Details

Structure
Approximately 63kD, IL-1 receptor family member, single pass type I membrane protein
Distribution

Mast cells, Th2 cells and fibroblasts

Function
Interacts with IL-33 and recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway
Interaction
Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor
IL-33
Cell Type
Mast cells, Th2, Fibroblasts
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
  2. Schmitt E, et al. 1990. Cytokine. 6:407.
  3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
  4. Takagi T, et al. 1993. Biochim. Biophys. Acta. 1178:194.
Gene ID
17082 View all products for this Gene ID
UniProt
View information about IL-33Ralpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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