PE anti-Nestin Antibody anti-Nestin

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Clone: 10C2 (See other available formats)
Regulatory Status: RUO
Other Names: NES
Isotype: Mouse IgG1, κ
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Product Citations: publications

10C2_PE_Nestin_Antibody_123115 — HUVEC cells were treated with the True-Nuclear™ Transcription Factor Buffer Set, then stained with Nestin (clone 10C2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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656805

25 tests

123€

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656806

100 tests

268€

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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_2566381 (BioLegend Cat. No. 656805)
AB_2566381 (BioLegend Cat. No. 656806)

Antigen Details

Structure: 1621 amino acids with predicted molecular weight of 200-220 kD
Distribution: Cytosol
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: 1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
Gene ID: 10763 View all products for this Gene ID
UniProt: View information about Nestin on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Nestin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nestin	10C2	WB,ICC,IHC-P
Alexa Fluor® 594 anti-Nestin	10C2	ICC,IHC-P
Brilliant Violet 421™ anti-Nestin	10C2	ICC,IHC-P
PE anti-Nestin	10C2	ICFC
Alexa Fluor® 647 anti-Nestin	10C2	ICC,IHC-P
Alexa Fluor® 488 anti-Nestin	10C2	ICC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 09/12/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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