- Clone
- GHI/75 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V B032
- Other Names
- ILT2, LIR-1, LILRB1, CD85
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![GHI_75_PECyanine5_CD85j_Antibody_101223 GHI_75_PECyanine5_CD85j_Antibody_101223](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/GHI_75_PECyanine5_CD85j_Antibody_101223.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human CD85j (ILT2) (clone GHI/75) PE/Cyanine5 (left) or mouse IgG2b, κ PE/Cyanine5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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333731 | 25 tests | 128€ | ||||
333732 | 100 tests | 292€ |
CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85j is the 110kD member, known as ILT2, LIR1, or LILRB1, and MIR7. ILT2 structurally has four Ig domains and contains ITIMs in its cytoplasmic tail that provide inhibitory signals by recruiting SHP-1. ILT2 is found on the surface of B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells. The ligands of ILT2 include a broad range of HLA-A, -B molecules, some HLA-C and HLA-G molecules, and the human cytomegalovirus UL18 protein.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported application: Block HLA-G induced TGF-ß1 production.
- Application References
-
- Pulford K, et al. 1991. Clin. Exp. Immunol. 85:429
- Mclntire RH, et al. 2004. J. Leukoc. Biol. 76:1220
- RRID
-
AB_3083316 (BioLegend Cat. No. 333731)
AB_3083316 (BioLegend Cat. No. 333732)
Antigen Details
- Structure
- ILT/LIR family, Ig superfamily, 110 kD
- Distribution
-
B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells.
- Function
- Inhibitory receptor for MHC class I
- Ligand/Receptor
- HLA-A, -B, some HLA-C and G molecules, human cytomegalovirus UL18 protein
- Cell Type
- B cells, Dendritic cells, Monocytes, NK cells, Plasma cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006 - Gene ID
- 10859 View all products for this Gene ID
- UniProt
- View information about CD85j on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD85j Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD85j (ILT2) | GHI/75 | FC,FA |
Biotin anti-human CD85j (ILT2) | GHI/75 | FC |
PE anti-human CD85j (ILT2) | GHI/75 | FC |
Alexa Fluor® 647 anti-human CD85j (ILT2) | GHI/75 | FC |
PE/Cyanine7 anti-human CD85j (ILT2) | GHI/75 | FC |
PerCP/Cyanine5.5 anti-human CD85j (ILT2) | GHI/75 | FC |
APC anti-human CD85j (ILT2) | GHI/75 | FC |
APC/Fire™ 750 anti-human CD85j (ILT2) | GHI/75 | FC |
PE/Dazzle™ 594 anti-human CD85j (ILT2) | GHI/75 | FC |
Ultra-LEAF™ Purified anti-human CD85j (ILT2) | GHI/75 | FC,FA |
TotalSeq™-C0896 anti-human CD85j (ILT2) | GHI/75 | PG |
TotalSeq™-A0896 anti-human CD85j (ILT2) | GHI/75 | PG |
TotalSeq™-B0896 anti-human CD85j (ILT2) | GHI/75 | PG |
FITC anti-human CD85j (ILT2) Antibody | GHI/75 | FC |
PE/Cyanine5 anti-human CD85j (ILT2) | GHI/75 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD85j (ILT2)
Human peripheral blood lymphocytes stained with purified GHI... -
Biotin anti-human CD85j (ILT2)
Human peripheral blood lymphocytes stained with biotinylated... -
PE anti-human CD85j (ILT2)
Human peripheral blood lymphocytes stained with GHI/75 PE -
Alexa Fluor® 647 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes stained with GHI/75 Alexa... -
PE/Cyanine7 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with CD85j (... -
PerCP/Cyanine5.5 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with CD85j (... -
APC anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with CD85j (... -
APC/Fire™ 750 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with CD85j (... -
PE/Dazzle™ 594 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with CD85j (... -
Ultra-LEAF™ Purified anti-human CD85j (ILT2)
Human peripheral blood lymphocytes stained with LEAF™ purifi... -
TotalSeq™-C0896 anti-human CD85j (ILT2)
-
TotalSeq™-A0896 anti-human CD85j (ILT2)
-
TotalSeq™-B0896 anti-human CD85j (ILT2)
-
FITC anti-human CD85j (ILT2) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine5 anti-human CD85j (ILT2)
Human peripheral blood lymphocytes were stained with anti-hu...
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