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PE/Cyanine7 anti-mouse CD93 (AA4.1, early B lineage) Antibody

Pricing & Availability
Clone
AA4.1 (See other available formats)
Regulatory Status
RUO
Other Names
AA4.1, C1qRp, Early B lineage antigen
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
AA4-1_PECyanine7_CD93_Antibody_1_081424
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45R/B220 (clone RA3-6B2) FITC and anti-mouse CD93 (clone AA4.1) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
  • AA4-1_PECyanine7_CD93_Antibody_1_081424
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45R/B220 (clone RA3-6B2) FITC and anti-mouse CD93 (clone AA4.1) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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136505 25 µg 90€
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136506 100 µg 268€
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Description

CD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo, indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Pre-B lymphoma 70Z/3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. McKearn JP, et al. 1984. J. Immunol. 132:332
  2. Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed
Product Citations
  1. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  2. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  3. Delgado-Benito V, et al. 2020. J Exp Med. 217:00:00. PubMed
  4. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
  5. Barajas‐Mora EM et al. 2018. Molecular cell. 73(1):48-60 . PubMed
  6. Lam WY et al. 2018. Cell reports. 24(9):2479-2492 . PubMed
  7. Li H, et al. 2020. Nature. 584:274. PubMed
  8. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  9. Jacoby E, et al. 2016. Nat Commun. 7:12320. PubMed
  10. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  11. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  12. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  13. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  14. Yamada H, et al. 2018. J Immunol. 201:3492. PubMed
  15. Zhang B, et al. 2021. Nature. 599:471. PubMed
  16. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
RRID
AB_2044011 (BioLegend Cat. No. 136505)
AB_2044011 (BioLegend Cat. No. 136506)

Antigen Details

Structure
A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.
Distribution

Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.

Function
CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133
2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895
3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406
4. Li YS, et al. 1996. Immunity 5:527
5. Szilvassy SJ, et al. 1993. Blood 81:2310

Gene ID
17064 View all products for this Gene ID
UniProt
View information about CD93 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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