PE/Fire™ 744 anti-human CD127 (IL-7Rα) Antibody, CD127, A019D5

Pricing & Availability

Clone: A019D5 (See other available formats)
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

A019D5_PE-Fire-744_-CD127_FC-1_072224 — Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD127 (IL-7Rα) (clone A019D5) PE/Fire™ 744 (left) or mouse IgG1, κ PE/Fire™ 744 isotype control (right).

Compare all formats See PE/Fire™ 744 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

351377

25 tests

136€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

351378

100 tests

328€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD127
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) application: proteogenomics¹.
RRID: AB_3662253 (BioLegend Cat. No. 351377)
AB_3662253 (BioLegend Cat. No. 351378)

Antigen Details

Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD127 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD127 (IL-7Rα)	A019D5	FC
PE anti-human CD127 (IL-7Rα)	A019D5	FC
Pacific Blue™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)	A019D5	FC
FITC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)	A019D5	FC
APC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)	A019D5	FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)	A019D5	FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα)	A019D5	FC
Biotin anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα)	A019D5	PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)	A019D5	FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
Spark YG™ 581 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα)	A019D5	PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
APC/Fire™ 750 anti-human CD127	A019D5	FC
PE anti-human CD127	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127	A019D5	FC
PE/Cyanine7 anti-human CD127	A019D5	FC
Spark Red™ 718 anti-human CD127 (IL-7Rα)	A019D5	FC
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 744 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Fire™ 780 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Fire™ 806 anti-human CD127 (IL-7Rα)	A019D5	FC
Spark PLUS B550™ anti-human CD127 (IL-7Rα)	A019D5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.