PE/Fire™ 744 anti-human CD25 Antibody, CD25, M-A251

Pricing & Availability

Clone: M-A251 (See other available formats)
Regulatory Status: RUO
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ
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Product Citations: publications

A. M-A251_PE-FIRE-744_CD25_1_112223 — PHA-stimulated (3 days) human peripheral blood mononuclear cells were stained with anti-human CD4 (clone SK3) FITC and with anti-human CD25 (clone M-A251) PE/Fire™ 744 (left) or mouse IgG1, κ PE/Fire™ 744 isotype control (right).

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356161

25 tests

108€

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356162

100 tests

276€

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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PHA-induced lymphocyte cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat. No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections¹ and spatial biology (IBEX)^2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
RRID: AB_3097417 (BioLegend Cat. No. 356161)
AB_3097417 (BioLegend Cat. No. 356162)

Antigen Details

Structure: Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution: Activated T and B cells, monocytes/macrophages, Tregs
Interaction: Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Spark YG™ 581 anti-human CD25	M-A251	FC
PE/Fire™ 640 anti-human CD25 Antibody	M-A251	FC
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PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
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PE/Cyanine7 anti-human CD25	M-A251	FC
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PE/Fire™ 744 anti-human CD25	M-A251	FC
PerCP/Fire™ 806 anti-human CD25	M-A251	FC
Brilliant Violet 650™ anti-human CD25	M-A251	FC
Brilliant Violet 750™ anti-human CD25	M-A251	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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