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PerCP anti-human CD203c (E-NPP3) Antibody

Pricing & Availability
Clone
NP4D6 (See other available formats)
Regulatory Status
RUO
Workshop
HLDA8
Other Names
E-NPP3, ENPP3
Isotype
Mouse IgG1, κ
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Product Citations
publications
NP4D6_PerCPCy55_1_052412
Overnight cultured human peripheral blood mononuclear cells were stained with anti-human IgE APC and CD203c (clone NP4D6) PerCP (top), or mouse IgG1, κ PerCP isotype control (bottom).
  • NP4D6_PerCPCy55_1_052412
    Overnight cultured human peripheral blood mononuclear cells were stained with anti-human IgE APC and CD203c (clone NP4D6) PerCP (top), or mouse IgG1, κ PerCP isotype control (bottom).
  • NP4D6_PerCPCy55_2_052412
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324616 100 tests 268€
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Description

CD203c, a transmembrane protein and a member of the ectoenzyme family, is involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity). The molecular weight of CD203c is between 130 and 150 kD under reducing conditions and 270 kD under non-reducing conditions. CD203c is expressed on basophils and mast cells, and is highly expressed on activated basophils. Secretory glands in endometrium and glioma cells are also positive. CD203c is a multifunctional ectoenzyme involved in the clearance of extracellular nucleotides whose substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HEK-293 cells transfected with human E-NPP3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application References
  1. Bühring HJ, et al. 1999. Blood 94:2343.
  2. Bühring HJ, et al. 2001. Blood 97:3303.
  3. Platz IJ, et al. 2001. Int. Arch. Allergy Immunol. 126:335.
  4. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  5. Gernez Y, et al. 2011. Int. Arch. Allergy Immunol. 154:318. (FC) PubMed
RRID
AB_11218606 (BioLegend Cat. No. 324616)

Antigen Details

Structure
Transmembrane ectoenzyme family member involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity); reduced molecular weight is 130 and 150 kD, unreduced is 270 kD
Distribution

Basophils and mast cells, highly expressed on activated basophils; secretory glands in endometrium and glioma cells are also positive

Function
Multifunctional ectoenzyme involved in the clearance of extracellular nucleotides
Ligand/Receptor
Substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD
Cell Type
Basophils, Mast cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Yano Y, et al. 2003. Int. J. Mol. Med. 12:763.
2. Andoh K, et al. 1999. Biochim. Biophys. Acta. 1446:213.

Gene ID
5169 View all products for this Gene ID
UniProt
View information about CD203c on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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