PerCP/Cyanine5.5 anti-human CD146 MUC18, Mel-CAM Antibody anti-CD146

Pricing & Availability

Clone: SHM-57 (See other available formats)
Regulatory Status: RUO
Other Names: Muc-18, MCAM, Mel-CAM, S-endo
Isotype: Mouse IgG2a, κ
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Product Citations: publications

SHM-57_PerCPCy5.5_051012 — Human cervical cancer cell line, HeLa, stained with purified CD146 (clone SHM-57) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).

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342014

100 tests

306€

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Description

CD146 is a 118 kD integral transmembrane glycoprotein also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
RRID: AB_11203721 (BioLegend Cat. No. 342014)

Antigen Details

Structure: 118 kD integral glycoprotein, Ig superfamily
Distribution: Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells
Function: Adhesion, monocytes transendothelial migration
Cell Type: Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Pickl WF, et al. 1997. J. Immunol. 158:2107
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746
Gene ID: 4162 View all products for this Gene ID
UniProt: View information about CD146 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD146 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PE anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PE/Cyanine7 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
APC anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PerCP/Cyanine5.5 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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