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- Clone
- O91A5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Annexin III, ANXA3, ANX3, Annexin-3, Inositol 1,2-Cyclic Phosphate 2-Phosphohydrolase, Placental Anticoagulant Protein III, 35-Alpha Calcimedin, Lipocortin III, PAP-III
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining with Purified anti-Annexin A3 (clone O91A5) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-Annexin A3 (clone O91A5) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm -
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-Annexin A3 (clone O91A5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 659951 | 25 µg | 124€ | ||||
| 659952 | 100 µg | 348€ | ||||
Annexin A3 (ANXA3) is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins that play diverse roles in cellular processes. In humans, Annexin A3 is primarily expressed in the liver, kidney, and various immune cells, where it participates in several physiological and pathological processes. Annexin A3 regulates membrane-related events, such as vesicle trafficking, exocytosis, and endocytosis. It is involved in the formation and stabilization of vesicular membranes, which is crucial for processes like the secretion of substances from cells and the recycling of membrane components. Annexin A3 is also implicated in anti-inflammatory responses. It can inhibit the synthesis of prostaglandins and leukotrienes, which are mediators of inflammation, by binding to and modulating enzymes involved in their production. In addition, Annexin A3 is involved in the differentiation and function of macrophages, key cells in the innate immune response. It influences processes such as phagocytosis, the engulfing of pathogens or debris by immune cells, and may affect the immune response to infections and tissue injury. Annexin A3 expression is often altered in various cancers, including liver, colorectal, and ovarian cancers. It can either promote or suppress tumor progression, depending on the context, by affecting processes like cell proliferation, apoptosis, and metastasis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of human Annexin A3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
This antibody is not suitable for immunocytochemistry (ICC).
Antigen Details
- Distribution
-
Expressed in blood, muscle, lung, liver, kidney etc. Localized in the cytoplasm and cell membrane
- Function
- Inhibitor of phospholipase A2 and cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate
- Interaction
- Binds to calcium and phospholipid
- Cell Type
- B cells, Lymphocytes, Monocytes, Neutrophils, Platelets
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure
- Antigen References
-
- Park J E, et al. 2005. Biochem Biophys. Res. Commun. 337:1283-7.
- Tong M, et al. 2015. Stem Cell Reports. 5:45-59.
- Wang L, et al. 2019. Cancer Sci. 110:1609-1620.
- Gene ID
- 306 View all products for this Gene ID
- UniProt
- View information about Annexin A3 on UniProt.org
Related FAQs
Other Formats
View All Annexin A3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Annexin A3 | O91A5 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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