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Purified anti-CDC73 Antibody

Pricing & Availability
Clone
W19164B (See other available formats)
Regulatory Status
RUO
Other Names
Parafibromin, HRPT2, Cell Division Cycle 73, HRPT1, PAF1/ RNA Polymerase II Complex Component. Hyperparathyroidism 2 Protein, C1orf28, FIHP, HYX
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19164B_PURE_CDC73_Antibody_1_020821
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-CDC73 antibody (clone W19164B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19164B_PURE_CDC73_Antibody_1_020821
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-CDC73 antibody (clone W19164B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19164B_PURE_CDC73_Antibody_2_020821
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, λ isotype ctrl antibody (Cat. No. 403801) or purified anti-CDC73 antibody (clone W19164B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of CDC73. Lane M: Molecular weight marker.
  • W19164B_PURE_CDC73_Antibody_3_020821
    HeLa cells (were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5 µg/mL (1:100 dilution) either purified rat IgG2b, λ isotype control antibody (Cat. No. 403801) (panel A) or purified anti-CDC73 antibody (clone W19164B) (panel B) overnight at 4 °C followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
941701 25 µg 95€
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941702 100 µg 235€
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Description

CDC73, also referred to as parafibromin or HRPT2, is a tumor-suppressor protein which plays a role in the suppression of epithelial-mesenchymal transition (EMT), inhibition of cell cycle progression and proliferation, and induction of apoptosis. In the nucleus, along with PAF1, LEO1, and CTR9, CDC73 forms the polymerase-associated factor (PAF) protein complex, which interacts with RNA polymerase II and the histone methyltransferase complex to control the expression of genes involved in cell cycle and metabolism, RNA polymerase II elongation, mRNA synthesis and processing, and chromatin and histone modifications. It also mediates IFN-γ signaling through interactions with JAK1 and JAK2. Loss of CDC73 function is associated with gastric carcinoma and mutations in CDC73 are linked to hyperparathyroidism-jaw tumor syndrome and parathyroid tumors. CDC73 plays a role in the coordinated activation and inactivation of Wnt, Hedgehog, and Notch signaling pathways through its role as a scaffold protein, where it sequesters the transcriptional factors β-catenin and GLI1. These interactions are dependent upon tyrosine phosphorylation of CDC73 mediated by PTK6 kinase or tyrosine dephosphorylation mediated by SHP2 phosphatase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human CDC73 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods were compatible with CDC73 staining.  

RRID
AB_2890860 (BioLegend Cat. No. 941701)
AB_2890860 (BioLegend Cat. No. 941702)

Antigen Details

Structure
CDC73 is a 531 amino acid protein with an expected molecular weight of 60 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Cell Cycle, Transcription, Wnt Signaling
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Kikuchi I, et al. 2016. Nat. Commun. 7:12887
2. Yart A, et al. 2005. Mol. Cell. Biol. 12:5052.
3. Zheng H, et al. 2017. Oncotarget. 8:95270.

Gene ID
79577 View all products for this Gene ID
UniProt
View information about CDC73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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