- Clone
- W15169A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ikaros family zinc finger protein 4 (IKZF4), KIAA1782, ZNFN1A4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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695302 | 100 µg | 231€ |
EOS, also known as IKZF4 (Ikaros family zinc finger 4), is a member of the Ikaros family of zinc finger transcription factors, which are key transcriptional repressors during development of lymphocytes. Each of the five Ikaros family members (Ikaros, Helios, Aiolos, Eos and Pegasus) has N-terminal and C-terminal zinc-finger motifs responsible for DNA binding and dimerization, respectively. EOS is a highly conserved DNA-binding protein that recognizes the typical 5'WGGAAT-3' Ikaros-binding sequence. EOS has been implicated in the development of lymphoid cells and nervous systems, as well as mediating FOXP3-dependent gene silencing in CD4+ regulatory T cells though interacting with FOXP3 and CTBP1.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human EOS recombinant protein (271-520 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:250 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W15169A does not cross-react with mouse (in-house tested). This antibody recognizes both isoforms of EOS and it also detects a non-specific protein of approximately 75 kD in the K562 cell line tested.
- RRID
-
AB_2715762 (BioLegend Cat. No. 695302)
Antigen Details
- Structure
- EOS has two isoforms. Isoform 1 has 585 amino acids with a predicted molecular weight of 64.1 kD. Isoform 2 has 544 amino acids with a predicted molecular weight of 59.7 kD. Both isoforms contain six zinc finger domains responsible for DNA-binding or prot
- Distribution
-
Nucleus
- Function
- EOS is a zinc finger transcription factor that is implicated in the development of lymphoid cells and nervous systems.
- Interaction
- EOS forms homodimers or heterodimers with other Ikaros family members. Interacts with CTBP2, SPI1, MITF, FOXP3, and CTBP1.
- Antigen References
-
1. Rieder SA, et al. 2015. J. Immunol. 195:553.
2. Raffin C, et al. 2013. J. Immunol. 191:4619.
3. Paradzik M, et al. 2012. Int. J. Immunopathol. Pharmacol. 25:893.
4. Bettini ML, et al. 2012. Immunity. 36:717.
5. John LB, Ward AC. 2011. Mol. Immunol. 48:1272.
6. Pan F, et al. 2009. Science. 325:1142. - Gene ID
- 64375 View all products for this Gene ID
- UniProt
- View information about EOS on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All EOS Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-EOS | W15169A | WB |
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Purified anti-EOS
Total protein from HeLa (negative control cell line), K562 a...
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