- Clone
- W18248A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Golgin A2, Golgin subfamily A member 2, 130 kD cis-Golgi matrix protein, Golgin 95, GOLGA2, SY11 Protein
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
937001 | 25 µg | 81€ | ||||
937002 | 100 µg | 203€ |
GM130 is a structural cis-Golgi protein that plays an essential role in vesicular transport. Mitotic fragmentation of the Golgi apparatus occurs through the CDK1-dependent regulation of the physical interaction between GM130 and the vesicle docking protein p115. Biochemical or genetic disruption of GM130 results in defective Golgi assembly. GM130 also plays a role in activating TPX2, a spindle assembly factor, to produce microtubule nuclei surrounding the Golgi, which are then directly bound by GM130 during mitotic events. Aberrant GM130 expression has been described in multiple malignancies where cells show altered Golgi structure and loss of polarity, lending to increased migration and invasion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human GM130 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not recognize mouse GM130 by western blot.
This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. Both methods were compatible with GM130 staining. - RRID
-
AB_2888895 (BioLegend Cat. No. 937001)
AB_2888895 (BioLegend Cat. No. 937002)
Antigen Details
- Distribution
-
Cis-Golgi/Ubiquitously expressed
- Function
- Golgi structural protein
- Biology Area
- Cell Biology, Cell Structure
- Molecular Family
- Microtubules, Organelle Markers
- Antigen References
-
1. Nakamura N, et al. 1997. Cell. 89:445.
2. Lowe M, et al. 1998. Cell. 94:783.
3. Wei JH, et al. 2015, Cell. 162:1. - Gene ID
- 2801 View all products for this Gene ID
- UniProt
- View information about GM130 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GM130 Antibody | W18248A | WB,ICC,IP |
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