Purified anti-Grp75 (Mortalin) Antibody (Previously Covance catalog# MMS-5164)

Pricing & Availability
Clone
N52A/42 (See other available formats)
Regulatory Status
RUO
Other Names
Stress-70 protein, mitochondrial, GRP-75, p66 MOT, C3H-specific antigen, heat shock protein 9A, heat shock protein, A, peptide-binding protein 74, heat shock 70 kD protein 9, heat shock protein cognate 74, 74 kD, A, 75 kD glucose-regulated protein
Previously
Covance Catalog# MMS-5164
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N52Aslash42_Purified_Grp75_Mortalin_Antibody_1_021918
Western blot of purified anti-Grp75 (Mortalin) antibody (clone N52A/42). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_1_021918
    Western blot of purified anti-Grp75 (Mortalin) antibody (clone N52A/42). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_2_021918
    IHC staining of purified anti-Grp75 (Mortalin) antibody (clone N52A/42) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_3_021918
    IHC staining of purified anti-Grp75 (Mortalin) antibody (clone N52A/42) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_4_021918
    IHC staining of purified anti-Grp75 (Mortalin) antibody (clone N52A/42) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_5_021918
    IHC staining of purified anti-Grp75 (Mortalin) antibody (clone N52A/42) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N52Aslash42_Purified_Grp75_Mortalin_Antibody_6_051519
    Western blot of purified anti-Grp75 (Mortalin) antibody (N52A/42). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
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818802 25 µL 67€
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818801 100 µL 132€
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Description

Glucose-regulated protein, 75 kD (Grp75), also known as heat shock protein 9A, Stress-70 protein, p66 MOT, and Mortalin, is an ubiquitously expressed chaperone protein that is expressed predominantly in mitochondria and is involved in mitochondrial localization, intracellular trafficking, and receptor internalization.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 551-766 of mouse SALM2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 1 - 5 µg/ml in human, mouse, and rat and 0.5 - 10 µg/ml in Drosophila.

Product Citations
  1. Blunsom NJ, et al. 2018. Biochim Biophys Acta. 1863:284. PubMed
RRID
AB_2564824 (BioLegend Cat. No. 818802)
AB_2564824 (BioLegend Cat. No. 818801)

Antigen Details

Structure
Expected MW: 70 kD
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Gene ID
15526 View all products for this Gene ID
UniProt
View information about Grp75 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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