Purified anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# MMS-101P)

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# MMS-101P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
636 publications
1_16B12_Purified_HA.11_Antibody_1_WB_061915
Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.
  • 1_16B12_Purified_HA.11_Antibody_1_WB_061915
    Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.
  • 2_16B12_PURE_HA-11_EpitopeTag_Antibody_120321
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with purified anti-HA.11 epitope Tag (clone 16B12) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by APC conjugated goat-anti-mouse IgG.
  • 3_16B12_Purified_HA.11_Antibody_2_ICC_081415
    Staining of Clone 16B12 on methanol fixed CHO cells transfected with an HA-tagged protein at 1 µg/ml. Cells were then incubated with Alexa Fluor® 594 Goat anti-mouse IgG secondary and were counterstained with DAPI (blue).
  • 4_16B12_Purified_HA.11_Antibody_2_WB_102317
    Immunoprecipitation of HA protein from CHO and CHO-HA stable cell extracts using HA Mouse primary antibody. Western blot analysis was performed using Direct-Blot™ HRP anti-HA (#901519). Direct-Blot™ HRP anti-β-actin (#643807) was used as a lysate loading control.
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901533 25 µL 48€
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901501 200 µL 285€
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901502 500 µL 445€
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901503 1 mL 677€
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested
FC, ICC, IP - Verified
Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:2,000-1:20,000. For immunocytochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
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Product Citations
  1. Wilhelm K, et al. 2016. Nature. 529:216-220. PubMed
  2. García R, et al. 2016. J Cell Sci. 129: 1649-1660. PubMed
  3. Matyskiela M, et al. 2016. Nature. 10.1038/nature18611. PubMed
  4. Radzisheuskaya A, et al. 2016. Nucleic Acids Res. 10.1093/nar/gkw583. PubMed
  5. Sun M, et al. 2016. J Biol Chem. 291: 21461 - 21473. PubMed
  6. Lu W, et al. 2016. Oncogene. 10.1038/onc.2016.300. PubMed
  7. Jenille Tan, Scott E. Martin 2016. PLoS One. 11:e0168968. PubMed
  8. Shimizu K, et al. 2017. Sci Signal. 10(460). PubMed
  9. Toulis V, et al. 2020. Cell Rep. 33:108360. PubMed
  10. Brumfield A, et al. 2020. Mol Biol Cell. mbcE20060356:. PubMed
  11. Diao L, et al. 2017. PLoS One. 10.1371/journal.pone.0180556. PubMed
  12. Zasadzińska E et al. 2018. Developmental cell. 47(3):348-362 . PubMed
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RRID
AB_2565005 (BioLegend Cat. No. 901533)
AB_2565005 (BioLegend Cat. No. 901501)
AB_2565005 (BioLegend Cat. No. 901502)
AB_2565005 (BioLegend Cat. No. 901503)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org
Go To Top Version: 10    Revision Date: 12/03/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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