- Clone
- 34-7.Rec (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BTF5, BT3.1, BTN3.1, BT3.2, BTF4, BTN3.2, BTF3, BTN3.3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
383202 | 100 µg | 216€ |
Butyrophilin subfamily 3A (BTN3A), also known as CD277, is a 56 kD type 1 transmembrane glycoprotein. The butyrophilin (BT)/B7 3A family includes three members, BTN3A1, BTN3A2, and BTN3A3, which share a highly conserved extracellular IgV-like domain. BTN3A is broadly expressed in various immune cells including T cells, B cells, NK cells, monocytes, dendritic cells, endothelial cells, and some tumor cells. It plays a role in T cell activation, regulating their proliferation and release of IFNƳ and other cytokines.
Clone 34-7.Rec has been shown to bind to a V region of BTN3A3 that is shared with BTN3A1 and BTN3A2.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- pRC-CMV-BTN3A3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product was formerly named “anti-human CD277 (BTN3A1).” The 34-7.Rec clone has been confirmed to recognize BTN3A3. Cross-reactivity with other BTN3A subfamily members has not been tested.
- RRID
-
AB_2924582 (BioLegend Cat. No. 383202)
Antigen Details
- Structure
- 56 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
- Distribution
-
Cell membrane
- Function
- Signaling receptor binding, Homodimerization
- Interaction
- TFF1, PEPL, BT3A2, G5E9A7
- Biology Area
- Immuno-Oncology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Tumor Suppressors
- Antigen References
-
- Vantourout P, et al. 2018. Proc Natl Acad Sci USA. 115:1039-44.
- Payne KK, et al. 2020. Science. 369: 942-9.
- Rigau M, et lat. 2020. Science. 367:eaay5516.
- Yang Y, et al. 2019. Immunity. 50:1043-53.
- Gene ID
- 11119 View all products for this Gene ID 10384 View all products for this Gene ID
- UniProt
- View information about CD277 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD277 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD277 (BTN3A) Recombinant Antibody | 34-7.Rec | FC |
APC anti-human CD277 (BTN3A) Recombinant Antibody | 34-7.Rec | FC |
PE/Cyanine7 anti-human CD277 (BTN3A) Recombinant Antibody | 34-7.Rec | FC |
PE anti-human CD277 (BTN3A) Recombinant Antibody | 34-7.Rec | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD277 (BTN3A) Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD277 (BTN3A) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD277 (BTN3A) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD277 (BTN3A) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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